VR1 N-Terminus (TRPV1)

VR1 (vanilloid receptor 1) is a channel activated by capsaicin, the pungent ingredient in hot peppers. It was isolated from sensory neurons. Sequence analysis suggests that VR1 has six transmembrane domains and intracellular N- and C-termini. It shares structural similarities with the family of putative store-operated calcium channels. VR1 is expressed by nociceptive sensory neurons. It is activated by noxious heat and is thought to function as transducer of noxious thermal stimuli.

Customer Data and Publications on VR1-N

Image: TRPV1 binds to the PI3K SH2 domains in a phosphotyrosine-independent manner. (A) In vitro interaction assay using TRPV1-FLAG from HEK293 cells (left) or recombinant N terminus of TRPV1 from bacteria with a FLAG epitope at its C-terminal end (right). FLAG-tagged TRPV1 protein was immobilized on anti-FLAG agarose beads, and recombinant GST fusion proteins corresponding to PI3K-p85 were tested for interaction. The bars in the cartoon represent the two independent clones of PI3K-p85ß identified in the yeast 2-hybrid assay. For the Western blot, equivalent amounts of input and bound proteins were probed with anti-GST antibody. (B) The recombinant N-terminal protein was not tyrosine phosphorylated. The recombinant FLAG-tagged protein was run in the left lane, and lysates from trkA-transfected HEK293 cells were run in the right lane. Duplicate gels were run, and Western blot analysis was then performed with either the anti-FLAG antibody (top) or the anti-phosphotyrosine antibody (bottom). (C) TRPV1 in HEK293 cells was not tyrosine phosphorylated. HEK293 cells expressing combinations of TRPV1, p75, and trkA, as indicated in the figure, were treated with either NGF or vehicle and then immunoprecipitated with anti-trkA (lanes 1–7) or anti-TRPV1 (lanes 8–13) antibodies. Lanes 1–7 were separated from lanes 8–13 and the membranes were probed with anti-trkA antibody and anti-TRPV1 antibody, respectively (top). Segments of the membrane were then reapposed for imaging. The membranes were then stripped and reprobed with anti-phosphotyrosine antibody (bottom). Published online Oct 30 2006. doi:10.1085/jgp.200609576
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