Mahendran Subramanian, Aimee‐Jayne Tyler, Eva Maria Luther, Elena Di Daniel, Jenson Lim and Jon Dobson. Oscillating Magnet Array−Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies. Nanomaterials 2017, 7, 28; doi:10.3390/nano7020028

...Primary rat hippocampal and cortical neurons were obtained from Neuromics (Edina, MN, USA) and disassociated using papain disassociation kit (Worthington, NJ, USA) according to the manufacturer’s instructions. Isolated neurons were maintained using neurobasal medium supplemented with 5% FBS, 0.5 mM Glutamax, 2% B27 supplement, 25 μM L‐glutamine and seeded onto poly‐D‐lysine–coated cells culture plates...

Juan Pablo Lezana1, Shachar Y. Dagan, Ari Robinson, Ron S. Goldstein, Mike Fainzilber, Francisca C. Bronfman, and Miguel Bronfman. Axonal pparγ promotes neuronal regeneration after injury. DOI: 10.1002/dneu.22353.

... Culture and regeneration analysis of human axons. NP1 human neural precursors were purchased from Neuromics (Minnesota, USA) and passaged up to 8 times before generation of neurons. Cells were used up until passage 10 after defrosting the purchased neural ...

James L. Miller*, Timothy J. Church, Dmitri Leonoudakis, Karen Lariosa-Willingham, Normand L. Frigon, Connie S, Tettenborn, Jeffrey R. Spencer, and Juha Punnonen. Discovery and Characterization of Nonpeptidyl Agonists of The Tissue-Protective Erythropoietin Receptor. Molecular Pharmacology. May 27, 2015 mol.115.098400.

...Cells were isolated from micro-surgically dissected embryonic day 18 rat cortices that were obtained from Neuromics (Edina, MN), and cultures were prepared according to the supplier's protocol. 

Jenica D. Tapocik, Estelle Barbier, Meghan Flanigan, Matthew Solomon, Alexandra Pincus, Andrew Pilling, Hui Sun, Jesse R. Schank, Courtney King, and Markus Heilig. microRNA-206 in Rat Medial Prefrontal Cortex Regulates BDNF Expression and Alcohol Drinking.The Journal of Neuroscience, 2 April 2014, 34(14): 5012-5022; doi: 10.1523/JNEUROSCI.3707-13.2014.

......and averaged across 3-5 well repetitions. Primary neuronal cultures. Primary rat cortical neurons were obtained from Neuromics and plated according to the company's instructions. Neurons were plated in 6-well plates coated with poly-d-lysine...

Mark RichardsChee Wee PhoonGwendoline Tze Wei GohEng Khuan SengXu Ming GuoCherine Mei Fong TanWoon-Khiong ChanJoel Mun Kin Lee. A New Class of Pluripotent Stem Cell Cytotoxic Small Molecules. Research Article | published 19 Mar 2014 | PLOS ONE 10.1371/journal.pone.0085039

...Primary hN2™ Human Neurons (Neuromics) were grown in hN2™ human neuron culture media (Neuromics)...

Yuji Kaneko, Hideki Shojo, Jack Burns, Meaghan Staples, Naoki Tajiri, Cesar V. Borlongan, DJ-1 ameliorates ischemic cell death in vitro possibly via mitochondrial pathway, Neurobiology of Disease, Available online 21 September 2013, ISSN 0969-9961, http://dx.doi.org/10.1016/j.nbd.2013.09.007.

...Cell culture and oxygen-glucose deprivation (OGD) hNPCs were obtained from Neuromics...

Alexzander Asea, Punit Kaur, Alexander Panossian, Karl Georg Wikman, Evaluation of molecular chaperons Hsp72 and neuropeptide Y as characteristic markers of adaptogenic activity of plant extracts. Phytomedicine, Available online 6 August 2013, ISSN 0944-7113, http://dx.doi.org/10.1016/j.phymed.2013.07.001

...using trypan blue exclusion test and routinely found to contain <5% dead cells. Primary human neurons were purchased from Neuromics (Edina, MN)...

Todd GK, Boosalis CA, Burzycki AA, Steinman MQ, Hester LD, et al. (2013) Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons. PLoS ONE 8(4): e58996. doi:10.1371/journal.pone.0058996

... a protocol that allows for culturing of E18 hippocampal neurons at high densities for more than 120 days. These cultured hippocampal neurons are (i) well differentiated with high numbers of synapses, (ii) anchored securely to their substrate, (iii) have high levels of functional connectivity, and (iv) form dense multi-layered cellular networks. We propose that our culture methodology is likely to be effective for multiple neuronal subtypes–particularly those that can be grown in Neurobasal/B27 media. This methodology presents new avenues for long-term functional studies in neurons...

Xiugong Gao, Hsiuling Lin, Radharaman Ray, Prabhati Ray. Toxicogenomic Studies of Human Neural Cells Following Exposure to Organophosphorus Chemical Warfare Nerve Agent VX. Neurochemical Research. February 2013.

...Human hN2 neurons were obtained from Neuromics...

Xiufang Guo, Severo Spradling, Maria Stancescu, Stephen Lambert, James J. Hickman. Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1. Biomaterials, In Press, Corrected Proof,Mar 2013.doi:10.1016/j.biomaterials.2013.02.061

...hNP1, were obtained from Neuromics (Edina, Minnesota)...

Wei Zhang , Radhia Benmohamed, Anthony C. Arvanites, Richard I. Morimoto, Robert J. Ferrante, Donald R. Kirsch, Richard B. Silverman. Cyclohexane 1,3-diones and their inhibition of mutant SOD1-dependent protein aggregation and toxicity in PC12 cells. Bioorganic & Medicinal Chemistry. Elsevier Ltd. All rights reserved.doi:10.1016/j.bmc.2011.11.039.

...Primary rat cortical tissue was purchased from Neuromics Inc., Edina, MN and used to initiate primary cortical neuron cultures. The tissue was isolated from micro-surgically dissected E18 embryonic Sprague/Dawley or Fischer 344 rat brain and shipped in a nutrient rich medium under refrigeration. To isolate neurons, the tissue was incubated with papain at a concentration of 2 mg/mL in Hibernate without calcium for 30 min at 37OC. The enzymatic solution was then removed, and 1 mL of culture media (Neurobasal, B27, 0.5 mM glutamine) was added. A sterile Pasteur pipette was used to gently disperse the cells, which were then washed, re-suspended and counted. The cells were plated on poly-D-lysine coated 96-well plates at a density of 20,000 cells/well and incubated at 37OC in a 5% CO2-humidified atmosphere for 5 days prior to use in compound testing. By microscopic inspection, the resulting cultures consisted of app. 90% neurons...

Majumder A, Dhara SK, Swetenburg R, Mithani M, Cao K, Medrzycki M, Fan Y, Stice SL. Inhibition of DNA methyltransferases and histone deacetylases induces astrocytic differentiation of neural progenitors. Stem Cell Res. 2013 Jul;11(1):574-86. doi: 10.1016/j.scr.2013.03.003. Epub 2013 Apr 2.

...Progenitor to Astrocytes Protocol: For astrocytic differentiation of hNP cells, neuronal differentiation media were supplemented with BMP2 (20 ng/mL) and combinations of Aza-C and TSA; Aza-C (500 nM), TSA (100 nM) and BMP2 (20 ng/mL) for 2 days, with one complete media change in between, followed by differentiation media supplemented with BMP2 but not with Aza-C or TSA. Cells were harvested prior to analysis at 5, 15 or 30 days of treatment or for cryopreservation at d6 or d10 of differentiation. For cryopreservation, cells were dissociated with Accutase™ and frozen in differentiation media containing10% DMSO. Viability was assessed at 30 days in Aza-C and TSA treated cultures by trypan blue exclusion, and datawas acquired using a Cellometer Auto T4® (Nexcelom Biosciences)...

Aparna Talekar, Antonello Pessi, and Matteo Porotto. Infection of primary neurons mediated by Nipah virus envelope proteins: Role of host target cells in antiviral action. J. Virol. doi:10.1128/JVI.00452-11.

...Hippocampus, Cortex and Ventricular Cells (Neuromics)...

A. Young, D.W. Machacek, S.K. Dhara, P.R. MacLeish, M. Benveniste, M.C. Dodla, C.D. Sturkie and S.L. Stice. Ion channels and ionotrophic receptors in a human embryonic stem cell derived neural progenitors. doi:10.1016/j.neuroscience.2011.04.039.

...mouse nonoclonal anti nestin (neuromics), mouse monoclonal anti tuj-1 (neuromics)...

Scott E. Counts and Elliott J. Mufson. Noradrenaline activation of neurotrophic pathways protects against neuronal amyloid toxicity. Journal of Neurochemistry. Volume 113 Issue 3, Pages 649 - 660. Published Online: 28 Jan 2010.

...Rat E18 primary hippocampal neurons were purchased from Neuromics (Edina, MN, USA) and cultured at ∼35 000/cm2 on poly-D-lysine using manufacturer protocols... 

Lisette T. Arnaud, Natura Myeku and Maria E. Figueiredo-Pereira. Proteasome–caspase–cathepsin sequence leading to tau pathology induced by prostaglandin J2 in neuronal cells. Journal of Neurochemistry. Volume 110 Issue 1, Pages 328 - 342 .

...Rat E18 cortical neuronal cultures were from Neuromics (Edina, MN)...

Karunya K. Kandimalla1, Olenych G. Scott, Smita Fulzele1, Michael W. Davidson, Joseph F. Poduslo. Mechanism of Neuronal versus Endothelial Cell Uptake of Alzheimer's Disease Amyloid β Protein. PLoS ONE 4(2): e4627. doi:10.1371/journal.pone.0004627.

... Rat primary hippocampal (RPH) neurons were isolated from the hippocampii of 18-day-old embryonic Sprague Dawley rat brains (Neuromics, Edina, MN). The hippocampii were dispersed using a fire polished Pasteur pipette and plated on poly-D-lysine (Sigma-Aldrich, St. Louis, MO) coated glass cover slips in B-27 neurobasal medium containing 0.5 mM glutamine and 25 µM glutamate (Invitrogen, Carlsbad, CA). The neuronal cells were grown under 5% CO2 in an incubator maintained at 37°C until differentiation...

Jennifer M Laing and Laure Aurelian. ΔRR vaccination protects from KA-induced seizures and neuronal loss through ICP 10PK-mediated modulation of the neuronal-microglial axis. Genetic

Mahendran Subramanian, Aimee‐Jayne Tyler 1,†, Eva Maria Luther 1, Elena Di Daniel 2,‡, Jenson Lim 3 and Jon Dobson Vaccines and Therapy 2008, 6:1doi:10.1186/1479-0556-6-1.

...Rat embryonic day 18 hippocampi were purchased from Neuromics (Edina, MN) and dissociated and plated at a density of 5 × 105 cells/dish on glass coverslips precoated with poly-L-Lysine (Sigma, St. Louis, MO) according to manufacturer's instruction. Over 99% of the cells stained with βIIITubulin antibody, indicating that they are neurons. The cultures were maintained in Neurobasal medium (Gibco-BRL) supplemented with B27 (Gibco-BRL)...

Shouqing Luo, Haruo Mizuta and David C. Rubinsztein. p21-activated kinase 1 promotes soluble mutant huntingtin self-interaction and enhances toxicity. Human Molecular Genetics 2008 17(6):895-905; doi:10.1093/hmg/ddm362

...Rat E18 cortical and striatal neurons were purchased from Neuromics (MN) or prepared from E18 rat brain tissues according to instructions from Neuromics, cultured in neurobasal (Invitrogen) medium supplemented with B27 (Invitrogen) and 0.5 mM glutamine and transfected with Lipofectamine 2000 according to standard methods...

 
...Micro-dissected hippocampal tissue was obtained from Neuromics...
 
Xiaoping Zhoua, Xiao-Wei Donga and Tony Priestley. The neuroleptic drug, fluphenazine, blocks neuronal voltage-gated sodium channels. doi:10.1016/j.brainres.2006.05.076
Copyright © 2006 Elsevier B.V. All rights reserved

... on voltage-clamped rat cerebrocortical neurons prepared from E18 rat embryos. Dissociated cortical neurons were purchased (Neuromics, Minnesota, USA; ...