Product Details
Catalog Number: 6-3304-002
Applications: Cell Assays
Type: Cell Isolation-Separation
Storage: Store all components at 2-8°C
Shipping: Ambient
Species Reactivity: Mouse
Downloads: Datasheet (pdf)
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1 Kit$187.00Add to Cart

Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD3 Fab-TACS® Agarose column Starter Kit allows label-free isolation of murine CD3+ T cells from splenocytes or other cell suspensions. CD3 is a co-receptor that associates with the T cell receptor and plays a role in T cell activation.

Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.

Advantages:

  • Highly pure cells directly from whole blood
  • Reversible binding to the target
  • Highly viable, non-activated cells
  • Label-free cells due to low affinity Fab fragments

Contents:

  • Strep-Tactin® TACS Agarose Columns
  • CD3 Fab-Strep, mouse
  • 10x Buffer CI
  • 100 mM Biotin stock solution
  • TACS Column Adapter
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