Whether it is a publication from a customer or through generated data, Neuromics is always looking to validate our reagents so that researchers can truly trust them. We've just received data (read here) to further demonstrate the trustworthiness of our human retinal microvascular endothelial cells (HRMECs) (cat.no. HEC09).
When culturing cells, consistency is necessary. It is impossible for researchers to rely on reagents that don't perform similarly when using an identical protocol.
To demonstrate the consistency of our HRMECs, we plated HRMECs in an 8-well chamber slide (a-h). The cells were stained with diluted primary antibodies CD31 (mouse anti-human BBA7 from R&D, 12 ug/ml) and von Willebrand factor (vWF) (rabbit anti-human from Abcam ab6994, 1:400). Both CD31 and vWF are used as markers of endothelial cells.
The results, shown in the images to the right (a-d in top image, e-h in bottom image), display that our endothelial cells are consistent in their expression of CD31 (green) and vWF (red). Overlap of expression of CD31 and vWF can be seen in the yellow color (expression of both green and red). While there is some variation in the number of cells co-expressing both CD31 and wWF, it falls within the expected range of statistical error.
Read more to view images of wells e-h...
More information on the data along with the protocol used to stain the cells is available here.
In addition to this consistency data, our HRMECs have been used by customers in published research many times (view all here). In the past year alone, the cells have been used in two separate diabetic retinopathy publications (learn more about the first and the second publication).
Along with our HRMECs, we have a wide offering of endothelial cells, check them all out here.