Neuromics has an impressive selection of human cells, most of which are unaltered primary cells. However, we also offer several GFP- and RFP-expressing cell types and lines. These cells enable new opportunities, applications, and ways to present results. We want to feature a publication released earlier this year that utilized our GFP-expressing human umbilical vein endothelial cells (GFP-HUVECs) (cat. GF01).
Image: GFP-HUVECs stained with VE-Cadherin and DAPI on an egg white based hydrogel.
Researchers at UCLA tested the feasibility of using egg white (EW) for tissue engineering in 3D bioprinting. EW has a history of being used as a substitute for other 3D culturing strategies such as gelatin, collagen, and Matrigel. The investigators employed our GFP-HUVECs to test whether an EW-based hydrogel would induce endothelialization in culture.
When culturing the cells, they found that GFP-HUVECs successfully adhere, grow, and form junctions on EW hydrogels. Furthermore, GFP-HUVECs spread better on the EW hydrogels than on gelatin methacryloyl (GelMA) and Matrigel. Altogether, they conclude that egg whites are promising tissue generation and 3D culturing tools, but further research is needed. You can read the complete study here.
Bottom image: Fluorescent imaging of GFP-HUVECs on days 1-4 seeded in various hydrogels.
As mentioned in our introduction, Neuromics offers several GFP- and RFP-expressing cells, including cancer cell lines, CNS cells, fibroblasts, and more. You can explore all our human cells here, and you can explore how researchers cite them here.