The identification and production of monoclonal antibodies is essential for investigating immune responses and developing therapies. In addition, they can be utilized for the direct and indirect detection of antigens in various assays, as capture molecule immobilized on microplates, or as ligand for protein purification, making them a versatile tool in biotechnology.

Different methods were developed for monoclonal antibody discovery including hybridoma technology, phage display or single B cell sorting.

Their common aim is the identification of high affinity antibodies that recognize a specific antigen of choice. This makes the antigen a central component for antibody development. Fusing a Twin-Strep-tag® to an antigen helps to make this central component applicable for various steps in the development process.

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