Product Details
Catalog Number: 6-5000-001
Applications: FACS
Type: Fluoro-Stain
Format A: liquid
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
10 x for 0.5-1 x 10^8 cells$3,360.00Add to Cart
3 x for 0.5-1 x 10^8 cells$1,128.00Add to Cart
For 0.5-1 x 10^8 cells$396.00Add to Cart

*Cat.No. 6-5000-001 (previously called Strep-Tactin® PE for MHC I Streptamers) replaces Cat. No. 6-5001-005 - Strep-Tactin® PE for Fab Streptamers. The products are completely identical.

For fluorescence-staining of cell isolates obtained after magnetic Fab Streptamer cell isolation. All required reagents, except Strep-Tactin PE or APC, are included in the Fab Streptamer isolation kits.

Streptamers, are used for efficient staining or isolation of cells. After separation of the labeled cells from non-labeled cells by flow-cytometric or magnetic cell isolation, the Streptamers are efficiently disrupted on the cell by addition of biotin. Subsequently, the dissociation and removal of the Strep-Tactin® backbone leaves monomeric Fab-Strep proteins on the surface of the cell.

Images

Receptor specific target cells are first labeled with fluorescent Fab Streptamer® (complexes of Fab-Streps and Strep-Tactin® PE or APC). In a second step, the isolated cells are liberated from all labeling reagents by addition of D-biotin (vitamin H) and washing in order to obtain a functional, completely label-free receptor specific cell preparation.

Receptor specific target cells are first labeled with fluorescent Fab Streptamer® (complexes of Fab-Streps and Strep-Tactin® PE or APC). In a second step, the isolated cells are liberated from all labeling reagents by addition of D-biotin (vitamin H) and washing in order to obtain a functional, completely label-free receptor specific cell preparation.