These expression vectors offer rapid and highly efficient subcloning of an arbitrary gene – initially cloned into a Donor Vector - to simultaneously fuse it with many different genetic surroundings via transfer into Acceptor Vectors to generate Destination Vectors. The latter enable the efficient expression of your protein with various features (e.g. different tags and different promoters) in different hosts.
Our selection of expression vectors include E. coli expression vectors (pASG-IBA and pASK-IBA vectors), mammalian vectors (pDSG-IBA, pESG-IBA, and pCSG-IBA vectors), yeast expression vectors (pYSG-IBA vectors), Baculo virus vectors (pLSG-IBA vectors), and StarGate entry vectors (pENTRY-IBA51).
We also have a MEXi product portfolio for use in mammalian protein expression, including a HEK293/EBNA-1 cell line and cell culture media.
- pASG-IBA vectors
pASG-IBA vectors are E. coli expression vectors with the following features:
- High-level expression in E. coli, also for toxic proteins
- Tightly regulated expression due to anhydrotetracycline (AHT) inducible tetA promoter/operator
- Option for periplasmic expression due to ompA signal sequence
- No catabolite repression - no influence of medium components
- Not influenced by the genetic background – wide choice of E. coli expression strains
- Inexpensive induction with AHT.
Name Catalog # Type Species Applications Size Price Competent cells E. coli TOP10 5-1600-020 20 rxns $185.90 pASG-IBA102 5-4102-001 Expression Vector E. Coli Gene Transfer 5 ug $358.60 pASG-IBA103 5-4103-001 Expression Vector E. Coli Gene Transfer 5 ug $358.60 pASG-IBA104 5-4104-001 Expression Vector E. Coli Gene Transfer 5 ug $358.60 pASG-IBA105 5-4105-001 Expression Vector E. Coli Gene Transfer 5 ug $358.60 pASG-IBA143 5-4143-001 Expression Vector E. Coli Gene Transfer 5 ug $358.60 pASG-IBA144 5-4144-001 Expression Vector E. Coli Gene Transfer 5 ug $358.60 pASG-IBA145 5-4145-001 Expression Vector E. Coli Gene Transfer 5 ug $358.60 pASG-IBA2 vector 5-4002-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBA23 vector 5-4023-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBA3 vector 5-4003-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBA4 vector 5-4004-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBA43 vector 5-4043-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBA44 vector 5-4044-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBA45 vector 5-4045-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBA5 vector 5-4005-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBA62 vector 5-4062-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBA64 vector 5-4064-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBAwt1 vector 5-4000-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 pASG-IBAwt2 vector 5-4001-001 Expression Vector E. Coli Gene Transfer 5 µg $358.60 - pASK-IBA vectors
pASK-IBA vectors are expression vectors for recombinant protein expression in E. coli, vectors with the Tet promoter (pASK-IBA), protease cleavage site (Factor Xa) and with chloramphenicol resistance gene are available.
Name Catalog # Type Species Applications Size Price Competent cells E. coli TOP10 5-1600-020 20 rxns $185.90 pASK-IBA2C 2-1321-000 Expression Vector E. Coli Targeted Protein Expression 5 ug $310.20 pASK-IBA3C 2-1322-000 Expression Vector E. Coli Targeted Protein Expression 5 ug $310.20 pASK-IBA4C 2-1323-000 Expression Vector E. Coli Targeted Protein Expression 5 ug $310.20 pASK-IBA5C 2-1324-000 Expression Vector E. Coli Targeted Protein Expression 5 ug $310.20 pASK-IBA6C 2-1325-000 Expression Vector E. Coli Targeted Protein Expression 5 ug $310.20 pASK-IBA7C 2-1326-000 Expression Vector E. Coli Targeted Protein Expression 5 ug $310.20 - pDSG-IBA vectors
pDSG-IBA vectors are mammalian expression vectors with the following features:
- CMV promoter: high level constitutive expression
- oriP: origin of replication from Epstein-Barr Virus for extrachromosomal replication driven by EBNA-1 (MEXi 293E cells express EBNA-1)
- ColE1 + Amp: for propagation in E. coli
- BM40: secretion signal to transfer the expressed protein into the medium (not on all vectors available)
Name Catalog # Type Species Applications Size Price Competent cells E. coli TOP10 5-1600-020 20 rxns $185.90 pDSG-IBA102 vector 5-5219-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pDSG-IBA103 vector 5-5220-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pDSG-IBA104 vector 5-5221-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pDSG-IBA105 vector 5-5222-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pDSG-IBAwt1 vector 5-5200-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pDSG-IBAwt2 vector 5-5201-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 - pESG-IBA vectors
pESG-IBA vectors are mammalian expression vectors with the following features:
- CMV promoter: high level constitutive expression
- SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
- ColE1 + Amp: for propagation in E. coli
- NeoR: neomycin resistance gene to generate stable cell lines
- BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
Name Catalog # Type Species Applications Size Price Competent cells E. coli TOP10 5-1600-020 20 rxns $185.90 pESG-IBA102 vector 5-4502-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pESG-IBA103 vector 5-4503-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pESG-IBA104 vector 5-4504-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pESG-IBA105 vector 5-4505-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pESG-IBA3 vector 5-4403-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pESG-IBA5 vector 5-4405-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pESG-IBAwt1 vector 5-4400-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pESG-IBAwt2 vector 5-4401-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 - pCSG-IBA vectors
pCSG-IBA vectors are mammalian expression vectors with the following features:
- CMV promoter: high level constitutive expression
- oriP: origin of replication from Epstein Barr Virus for extrachromosomal replication driven by EBNA-1
- EBNA-1 gene: Expression of EBNA-1 for initiation of extrachromosomal replication by oriP
- SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
- NeoR: neomycin resistance gene to generate stable cell lines
- ColE1 + Amp: for propagation in E. coli
- BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)
Name Catalog # Type Species Applications Size Price Competent cells E. coli TOP10 5-1600-020 20 rxns $185.90 pCSG-IBA102 vector 5-5102-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pCSG-IBA103 vector 5-5103-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pCSG-IBA104 vector 5-5104-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pCSG-IBA105 vector 5-5105-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pCSG-IBAwt1 vector 5-5000-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 pCSG-IBAwt2 vector 5-5001-001 Expression Vector Mammalian Gene Transfer 5 µg $358.60 - pYSG-IBA vectors
pYSG-IBA vectors are yeast expression vectors with the following features:
- High-level expression in yeast
- Tightly regulated expression due to Cu++-inducible CUP1 promoter
- Ampicillin resistance gene and markers leu2-d and URA3
- High copy number
Name Catalog # Type Species Applications Size Price Competent cells E. coli TOP10 5-1600-020 20 rxns $185.90 - pLSG-IBA vectors
pLSG-IBA vectors are baculo virus expression vectors with the following features:
- Cloning and expression of recombinant proteins in insect cells
- Polyhedrin promoter drives high-level expression of the insert
- pUC ori and ampicillin resistance gene support propagation in E. coli
Name Catalog # Type Species Applications Size Price Competent cells E. coli TOP10 5-1600-020 20 rxns $185.90 - pENTRY-IBA vectors
StarGate Entry Vector is required to generate Donor Vectors for subsequent Destination Vector generation. The vector encodes a kanamycin resistance gene.
Name Catalog # Type Species Applications Size Price Competent cells E. coli TOP10 5-1600-020 20 rxns $185.90 StarGate ENTRY Vector pENTRY-IBA51 5-4091-001 Expression Vector Gene Transfer 5 µg $358.60 - MEXi Products
We also have a MEXi product portfolio for use in mammalian protein expression, including a HEK293/EBNA-1 cell line and cell culture media.
Name Catalog # Type Species Applications Size Price MEXi-293E cells 2-6001-001 Primary Cells Mammalian 1.5 ml $645.70 MEXi-CM 2-6010-010 Cell Growth Media Cell Assays 1000 ml $171.60 MEXi-TM 2-6011-010 Cell Growth Media Cell Assays 1000 ml $171.60