The pASG-IBA102 vector allows the expression of Twin-Strep-tag®-fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, the Twin-Strep-tag® for C-terminal fusion to the recombinant protein and the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli.
StarGate Acceptor Vector for bacterial expression.
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