Membrane proteins require special attention. The removal of the cell membrane is usually required for many protein assays. Over time scientists have developed multiple approaches to solubilize & stabilize membrane proteins outside the membrane. Cube Biotech offers Ultrapure detergents, MSP Nanodiscs and Copolymer Nanodiscs.
Know your protein of interest? Consult the Cube Solubilization Database for recommendations on which copolymer to use. Otherwise, we recommend the screening kit.
Need to purify your protein? Cube recommends working with IBA Lifesciences' MagStrep Strep-Tactin®XT Beads, also distributed by Neuromics.
- Copolymer Nanodisc Products
Copolymer nanodiscs are the most flexible approach to the solubilization & stabilization of membrane proteins. Their greatest advantage lies in the fact that they stabilize the membrane protein in its native lipid environment.
Cubipol
DIBMA
DIBMAs or Diisobutylene-maleic acids are alternatives to detergents which are normally used in membrane protein solubilization. With DIMBAs, you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization.
SMA
Styrene maleic anhydride (SMA) belongs to a group of synthetic polymers that can form synthetic nanodiscs. These small disc-shaped complexes consist of a polymer, phospholipids of the cell membrane, and a membrane protein. They enable membrane protein stabilization & solubilization without the use of detergents.
Name Catalog # Type Species Applications Size Price NativeMP™ Screening Kit 18298-CUBE 30 x 1 x 50 mg $2,471.00 SMALP 140 18220-CUBE 10 x 50 mg $615.00 SMALP 140 18221-CUBE 1 g $1,089.00 SMALP 140 18222-CUBE 10 g $10,415.00 SMALP 140-I 18230-CUBE 10 x 50 mg $615.00 SMALP 140-I 18231-CUBE 1 g $1,117.00 SMALP 140-I 18232-CUBE 10 g $8,369.00 SMALP 200 18210-CUBE 10 x 50 mg $615.00 SMALP 200 18211-CUBE 1 g $1,089.00 SMALP 200 18212-CUBE 10 g $10,415.00 SMALP 300 18200-CUBE 10 x 50 mg $615.00 SMALP 300 18201-CUBE 1 g $1,089.00 SMALP 300 18202-CUBE 10 g $10,415.00 SMALP BZ Screening Kit 16690-CUBE 4 x 3 x 50 mg $866.00 SMALP BZ25 18611-CUBE 10 x 50 mg $615.00 SMALP BZ25 18612-CUBE 1 g $1,089.00 SMALP BZ25 18613-CUBE 10 x 1 g $10,415.00 SMALP BZ30 18621-CUBE 10 x 50 mg $615.00 SMALP BZ30 18622-CUBE 1 g $1,089.00 SMALP BZ30 18623-CUBE 10 x 1 g $10,415.00 SMALP BZ35 18633-CUBE 10 x 1 g $10,415.00 SMALP BZ35 18631-CUBE 10 x 50 mg $615.00 SMALP BZ35 18632-CUBE 1 g $1,089.00 SMALP BZ40 18643-CUBE 10 x 1 g $10,415.00 SMALP BZ40 18642-CUBE 1 g $1,089.00 SMALP BZ40 18641-CUBE 10 x 50 mg $615.00 SMALP Screening Set 18280-CUBE 4 x 3 x 50 mg $855.00 Sulfo-SMA 18523-CUBE 10 x 1 g $10,415.00 Sulfo-SMA 18522-CUBE 1 g $1,089.00 Sulfo-SMA 18521-CUBE 10 x 50 mg $615.00 AASTY
Poly(acrylic acid-co-styrene), AASTY or SAA, is a highly-alternating copolymer, well-suited for the generation of native lipid nanodiscs. The reactivity ratios of styrene and acrylic acid, allow for a more alternating monomer sequence in the polymer, than 2:1 SMA copolymers.
Ultrasolute™ Amphipol
Ultrasolute™ Amphipol differs from the old Amphipols by replacing linear octyl chains with hydrophobic, saturated cycles. This change improves the polymers' ability to extract membrane proteins from a biological membrane.
Name Catalog # Type Species Applications Size Price NativeMP™ Biotin Kit 19791-CUBE 6 x 1 x 50 mg $2,246.00 NativeMP™ Screening Kit 18298-CUBE 30 x 1 x 50 mg $2,471.00 Ultrasolute™ Amphipol 17 18301-CUBE 10 x 50 mg $615.00 Ultrasolute™ Amphipol 17 18302-CUBE 1 g $1,117.00 Ultrasolute™ Amphipol 17 18303-CUBE 10 x 1 g $8,369.00 Ultrasolute™ Amphipol 18 18321-CUBE 10 x 50 mg $615.00 Ultrasolute™ Amphipol 18 18322-CUBE 1 g $1,117.00 Ultrasolute™ Amphipol 18 18323-CUBE 10 x 1 g $8,369.00 Ultrasolute™ Amphipol 18 Biotin 19721-CUBE 10 x 50 mg $2,022.00 Ultrasolute™ Amphipol 18 Biotin 19722-CUBE 1 g $3,162.00 Ultrasolute™ Amphipol 18 Biotin 19723-CUBE 10 x 1 g $10,666.00 Copolymer Depletion
Synthetic copolymers are a great tool for the stabilization of membrane proteins outside the cell membrane.
Name Catalog # Type Species Applications Size Price PolyHunter Agarose (50 % Suspension) 19103-CUBE 10 ml $235.00 PolyHunter Agarose (50 % Suspension) 19107-CUBE 100 ml $1,118.00 PolyHunter Agarose (50 % Suspension) 19110-CUBE 250 ml $2,457.00 PolyHunter MagBeads (25 % Suspension) 19205-CUBE 5 ml $443.00 PolyHunter MagBeads (25 % Suspension) 19225-CUBE 25 ml $1,663.00 - MSP Nanodisc Products
Membrane scaffold proteins (MSPs) are typically used for the reconstitution of already isolated proteins. A variety of MSPs are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a His-tag, we recommend using untagged MSP proteins. For use in cell-free expression reactions, assembled nanodiscs are available.
Assembled Nanodiscs
Assembled nanodiscs are available as part of a Cube Biotech collaboration with the Institute of Biophysics at the University of Frankfurt. Bacterial and eucaryotic membrane proteins can be expressed, and activity may depend on the lipid composition of the assembled nanodisc.
MSP Proteins
Membrane scaffold proteins (MSPs) are typically used for the reconstitution of already isolated proteins. A variety of MSPs are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains.
Name Catalog # Type Species Applications Size Price - Ultrapure Detergents
Detergents are the most commonly used substances for membrane protein stabilization. Before you use a specific detergent for a new membrane protein you must screen for a matching one. For this purpose, we offer detergent screening sets.
Non-Ionic Maltosides
Name Catalog # Type Species Applications Size Price n-Decyl-Beta-Maltoside (DM) 16011-CUBE Protein Purification 5 x 5 g $2,945.00 n-Dodecyl-Beta-Maltoside (DDM) 16014-CUBE Protein Purification 5 x 1 g $792.00 Non-Ionic Glucosides
Name Catalog # Type Species Applications Size Price Octylglucopyranoside (OG) 16003-CUBE Protein Purification 5 x 5 g $1,618.00 Lipid-Like Detergents
Name Catalog # Type Species Applications Size Price n-Dodecyl-phosphocholine (Fos-choline-12) 16021-CUBE Protein Purification 1 g $374.00 n-Dodecyl-phosphocholine (Fos-choline-12) 16023-CUBE Protein Purification 5 x 5 g $6,335.00