Membrane proteins require special attention. The removal of the cell membrane is usually required for many protein assays. Over time scientists have developed multiple approaches to solubilize & stabilize membrane proteins outside the membrane. Cube Biotech offers Ultrapure detergents, MSP Nanodiscs and Copolymer Nanodiscs.
Know your protein of interest? Consult the Cube Solubilization Database for recommendations on which copolymer to use. Otherwise, we recommend the screening kit.
Need to purify your protein? Cube recommends working with IBA Lifesciences' MagStrep Strep-Tactin®XT Beads, also distributed by Neuromics.
- Copolymer Nanodisc Products
Copolymer nanodiscs are the most flexible approach to the solubilization & stabilization of membrane proteins. Their greatest advantage lies in the fact that they stabilize the membrane protein in its native lipid environment.
Cubipol
DIBMA
DIBMAs or Diisobutylene-maleic acids are alternatives to detergents which are normally used in membrane protein solubilization. With DIMBAs, you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization.
SMA
Styrene maleic anhydride (SMA) belongs to a group of synthetic polymers that can form synthetic nanodiscs. These small disc-shaped complexes consist of a polymer, phospholipids of the cell membrane, and a membrane protein. They enable membrane protein stabilization & solubilization without the use of detergents.
AASTY
Poly(acrylic acid-co-styrene), AASTY or SAA, is a highly-alternating copolymer, well-suited for the generation of native lipid nanodiscs. The reactivity ratios of styrene and acrylic acid, allow for a more alternating monomer sequence in the polymer, than 2:1 SMA copolymers.
Ultrasolute™ Amphipol
Ultrasolute™ Amphipol differs from the old Amphipols by replacing linear octyl chains with hydrophobic, saturated cycles. This change improves the polymers' ability to extract membrane proteins from a biological membrane.
Copolymer Depletion
Synthetic copolymers are a great tool for the stabilization of membrane proteins outside the cell membrane.
Name Catalog # Type Species Applications Size Price PolyHunter Agarose (50 % Suspension) 19103-CUBE 10 ml $196.00 PolyHunter Agarose (50 % Suspension) 19107-CUBE 100 ml $927.00 PolyHunter Agarose (50 % Suspension) 19110-CUBE 250 ml $2,035.00 PolyHunter MagBeads (25 % Suspension) 19205-CUBE 5 ml $368.00 PolyHunter MagBeads (25 % Suspension) 19225-CUBE 25 ml $1,378.00 - MSP Nanodisc Products
Membrane scaffold proteins (MSPs) are typically used for the reconstitution of already isolated proteins. A variety of MSPs are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a His-tag, we recommend using untagged MSP proteins. For use in cell-free expression reactions, assembled nanodiscs are available.
Assembled Nanodiscs
Assembled nanodiscs are available as part of a Cube Biotech collaboration with the Institute of Biophysics at the University of Frankfurt. Bacterial and eucaryotic membrane proteins can be expressed, and activity may depend on the lipid composition of the assembled nanodisc.
MSP Proteins
Membrane scaffold proteins (MSPs) are typically used for the reconstitution of already isolated proteins. A variety of MSPs are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains.
Nanodisc Assembly Kits
Nanodisc Assembly Kits provide labor-saving aliquots and protocols to generate empty nanodiscs. These are advantageous for use with cell-free protein expression reactions, where the nascent membrane proteins can insert directly into the nanodisc without preliminary detergent extraction.
Name Catalog # Type Species Applications Size Price Nanodisc Assembly Kit MSP1D1 dH5 DMPC 26241-CUBE Human 1 Kit $1,278.00 Nanodisc Assembly Kit MSP1D1 dH5 POPC 26243-CUBE Human 1 Kit $1,278.00 Nanodisc Assembly Kit MSP1D1 dH5-His DMPC 26221-CUBE Human 1 Kit $671.00 Nanodisc Assembly Kit MSP1D1 dH5-His POPC 26223-CUBE Human 1 Kit $671.00 Nanodisc Assembly Kit MSP1D1 DMPC 26231-CUBE Human 1 Kit $1,278.00 Nanodisc Assembly Kit MSP1D1 POPC 26233-CUBE Human 1 Kit $1,278.00 Nanodisc Assembly Kit MSP1D1-His DMPC 26211-CUBE Human 1 Kit $671.00 Nanodisc Assembly Kit MSP1D1-His POPC 26213-CUBE Human 1 Kit $671.00 Nanodisc Assembly Kit MSP1E3D1-His DMPC 26251-CUBE Human 1 Kit $671.00 Nanodisc Assembly Kit MSP1E3D1-His POPC 26253-CUBE Human 1 Kit $671.00 Nanodisc Assembly Kit MSP2N2-His DMPC 26271-CUBE Human 1 Kit $835.00 Nanodisc Assembly Kit MSP2N2-His POPC 26273-CUBE Human 1 Kit $835.00 - Ultrapure Detergents
Detergents are the most commonly used substances for membrane protein stabilization. Before you use a specific detergent for a new membrane protein you must screen for a matching one. For this purpose, we offer detergent screening sets.
Non-Ionic Maltosides
Name Catalog # Type Species Applications Size Price Detergent Screening Set 2 16094-CUBE Protein Purification 1 Set $693.00 Detergent Screening Set Classic 16092-CUBE Protein Purification 1 Set $564.00 n-Decyl-Beta-Maltoside (DM) 16010-CUBE Protein Purification 5 x 1 g $667.00 n-Decyl-Beta-Maltoside (DM) 16011-CUBE Protein Purification 5 x 5 g $2,561.00 n-Decyl-Beta-Maltoside (DM) 16012-CUBE Protein Purification 20 x 5 g $9,785.00 n-Dodecyl-Beta-Maltoside (DDM) 16014-CUBE Protein Purification 5 x 1 g $689.00 n-Dodecyl-Beta-Maltoside (DDM) 16015-CUBE Protein Purification 5 x 5 g $3,080.00 n-Dodecyl-Beta-Maltoside (DDM) 16016-CUBE Protein Purification 20 x 5 g $11,567.00 n-Nonyl-Beta-Maltoside (NM) 16065-CUBE Protein Purification 5 x 1 g $667.00 n-Nonyl-Beta-Maltoside (NM) 16066-CUBE Protein Purification 5 x 5 g $2,561.00 n-Tridecyl-Beta-Maltoside (TDM) 16074-CUBE Protein Purification 1 g $373.00 n-Tridecyl-Beta-Maltoside (TDM) 16075-CUBE Protein Purification 5 x 1 g $1,572.00 n-Tridecyl-Beta-Maltoside (TDM) 16076-CUBE Protein Purification 5 x 5 g $6,270.00 n-Undecyl-Beta-Maltoside (UDM) 16070-CUBE Protein Purification 5 x 1 g $667.00 n-Undecyl-Beta-Maltoside (UDM) 16071-CUBE Protein Purification 5 x 5 g $2,561.00 Non-Ionic Glucosides
Name Catalog # Type Species Applications Size Price Detergent Screening Set 2 16094-CUBE Protein Purification 1 Set $693.00 Detergent Screening Set Classic 16092-CUBE Protein Purification 1 Set $564.00 n-Nonyl-Beta-D-Glucopyranoside (NG) 16055-CUBE Protein Purification 5 x 1 g $667.00 n-Nonyl-Beta-D-Glucopyranoside (NG) 16056-CUBE Protein Purification 5 x 5 g $2,561.00 n-Octyl-1-Thio-Beta-D-Glucopyranoside (OTG) 16018-CUBE Protein Purification 5 x 1 g $397.00 n-Octyl-1-Thio-Beta-D-Glucopyranoside (OTG) 16019-CUBE Protein Purification 5 x 5 g $1,789.00 n-Octyl-1-Thio-Beta-D-Glucopyranoside (OTG) 16020-CUBE Protein Purification 20 x 5 g $7,147.00 Octylglucopyranoside (OG) 16002-CUBE Protein Purification 5 x 1 g $386.00 Octylglucopyranoside (OG) 16003-CUBE Protein Purification 5 x 5 g $1,407.00 Octylglucopyranoside (OG) 16004-CUBE Protein Purification 20 x 5 g $5,430.00 Lipid-Like Detergents