Affinity tags combined with a matching purification resin offer a great way to purify expressed proteins to homogeneity. Cube Biotech provides a broad range of resins, tags, and magbeads for you to choose from. Cube also offers activated resins and MagBeads for coupling to amino, thiol, and carboxy groups directly.
- IBA Lifesciences Protein Purification Products
- Protein Purification Kits
PureCube agarose resins are practical kits for your convenience. Equipped with columns, all necessary buffers, and of course, high-quality resins.
Name Catalog # Type Species Applications Size Price HisCube - Ni-INDIGO His-Tag Protein Purification MIDI Kit 80105-CUBE Protein Purification 1 Kit $1,272.00 HisCube - Ni-INDIGO His-Tag Protein Purification MINI Kit 80101-CUBE Protein Purification 1 Kit $1,329.00 HisCube - Ni-INDIGO MagBead Protein Purification MINI Kit 80201-CUBE Protein Purification 1 Kit $1,595.00 HisCube Binding Buffer 80181-CUBE Protein Purification 2 x 50 ml $178.00 HisCube Elution Buffer 80187-CUBE Protein Purification 50 ml $149.00 HisCube Wash Buffer 80184-CUBE Protein Purification 2 x 50 ml $178.00 PureCube 1-Step Batch Midi Plus 63208-CUBE 48 $755.00 PureCube 1-Step Batch Mini 63104-CUBE 50 $329.00 - His-Tag Affinity Resins & MagBeads
The polyhistidine tag is the most widely used protein affinity tag. Due to its small size of only six amino acids, it rarely interferes with the protein's function. Additionally, its low immunogenicity and versatility under native and denaturing conditions are highlighting this protein tag among others.
Ni-INDIGO
Cube Biotech introduces the novel Ni-INDIGO product line for His-tag protein purifications. The novel ligand INDIGO is loaded with nickel ions. Using the INDIGO ligand, purification of His-tagged proteins is possible in the presence of up to 20 mM DTT and 20 mM EDTA at high protein capacity.
Name Catalog # Type Species Applications Size Price HisCube - Ni-INDIGO His-Tag Protein Purification MIDI Kit 80105-CUBE Protein Purification 1 Kit $1,272.00 HisCube - Ni-INDIGO His-Tag Protein Purification MINI Kit 80101-CUBE Protein Purification 1 Kit $1,329.00 HisCube - Ni-INDIGO MagBead Protein Purification MINI Kit 80201-CUBE Protein Purification 1 Kit $1,595.00 PureCube 100 Compact Cartridge Ni-INDIGO 75302-CUBE Protein Purification 1 ml $134.00 PureCube 100 Compact Cartridge Ni-INDIGO 75306-CUBE Protein Purification 5 ml $447.00 PureCube 100 Ni-INDIGO Agarose 75103-CUBE Protein Purification 10 ml $316.00 PureCube 100 Ni-INDIGO Agarose 75105-CUBE Protein Purification 50 ml $1,267.00 PureCube 100 Ni-INDIGO Agarose 75110-CUBE Protein Purification 250 ml $5,457.00 PureCube Ni-INDIGO MagBeads 75201-CUBE Protein Purification 1 ml $208.00 PureCube Ni-INDIGO MagBeads 75205-CUBE Protein Purification 5 ml $875.00 PureCube Ni-INDIGO MagBeads 75225-CUBE Protein Purification 25 ml $3,277.00 Ni-NTA
The term Ni-NTA (Nickel NTA) refers to a nickel2+ ion that has been coupled to Nitrilotriacetic acid (NTA). Ni-NTA can then be coupled to agarose resin or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography).
Name Catalog # Type Species Applications Size Price PureCube 100 Compact Cartridge Ni-NTA 74302-CUBE Protein Purification 1 ml $126.00 PureCube 100 Compact Cartridge Ni-NTA 74306-CUBE Protein Purification 5 ml $418.00 PureCube 100 Ni-NTA Agarose 74103-CUBE Protein Purification 10 ml $281.00 PureCube 100 Ni-NTA Agarose 74105-CUBE Protein Purification 50 ml $964.00 PureCube 100 Ni-NTA Agarose 74110-CUBE Protein Purification 250 ml $4,147.00 PureCube Compact Cartridge Ni-NTA 31302-CUBE Protein Purification 1 ml $91.00 PureCube Compact Cartridge Ni-NTA 31306-CUBE Protein Purification 5 ml $273.00 PureCube Ni-NTA Agarose 31103-CUBE Protein Purification 10 ml $270.00 PureCube Ni-NTA Agarose 31105-CUBE Protein Purification 50 ml $919.00 PureCube Ni-NTA MagBeads 31201-CUBE Protein Purification 1 ml $186.00 PureCube Ni-NTA MagBeads 31205-CUBE Protein Purification 5 ml $795.00 PureCube Ni-NTA MagBeads 31225-CUBE Protein Purification 25 ml $2,960.00 Co-NTA
The term Co-NTA refers to a cobalt ion that has been coupled with Nitrilotriacetic acid. Co-NTA can then be coupled to agarose or magnetic beads for IMAC (Immobilized Metal Chelate Affinity Chromatography).
Name Catalog # Type Species Applications Size Price PureCube 100 Co-NTA Agarose 74405-CUBE Protein Purification 50 ml $1,050.00 PureCube 100 Co-NTA Agarose 74410-CUBE Protein Purification 250 ml $4,522.00 PureCube Co-NTA Agarose 31403-CUBE Protein Purification 10 ml $307.00 PureCube Co-NTA MagBeads 31505-CUBE Protein Purification 5 ml $854.00 PureCube Co-NTA MagBeads 31525-CUBE Protein Purification 25 ml $3,189.00 Ni-IDA
Ni-IDA is an abbreviation for a nickel ion that has been coupled to Iminodiacetic acid (IDA). In the field of protein purification, it is used for His-tag purifications.
Name Catalog # Type Species Applications Size Price PureCube Ni-IDA Agarose 30103-CUBE Protein Purification 10 ml $205.00 PureCube Ni-IDA MagBeads 30205-CUBE Protein Purification 5 ml $498.00 Cu-NTA
Cu-NTA is a term that refers to a copper ion that has been coupled to nitrilotriacetic acid (NTA). Cu-NTA can then be coupled to agarose or magnetic beads for Immobilized Metal Chelate Affinity Chromatography, short IMAC.
Name Catalog # Type Species Applications Size Price PureCube Cu-NTA MagBeads 31505-Cu-CUBE Protein Purification 5 ml $854.00 Zn-NTA
Zn-NTA is a term that refers to a zinc ion that has been coupled with nitrilotriacetic acid (NTA). Zn-NTA can then be coupled to agarose or magnetic beads for Immobilized Metal Chelate Affinity Chromatography, short IMAC.
Name Catalog # Type Species Applications Size Price PureCube Zn-NTA MagBeads 31505-Zn-CUBE Protein Purification 5 ml $854.00 PureCube Zn-NTA MagBeads 31525-Zn-CUBE Protein Purification 25 ml $3,189.00 - Custom IMAC Resins
Cube Biotech offers its high-quality NTA and IDA resin and MagBeads uncoupled to any metal ion. Choose the metal ion you want to couple.
Name Catalog # Type Species Applications Size Price PureCube NTA Agarose 31712-CUBE Protein Purification 500 ml $9,641.00 - Rho1D4-Tag Affinity Resins & MagBeads
Rho1D4, also called "1D4" is an affinity tag consisting of nine amino acids. The tag is preferably added to the C-Terminus of a protein of interest. Agarose resins and magnetic beads coupled with the matching Rho1D4-antibody are able to purify proteins that are tagged with Rho1D4. Rho1D4 protein purification assays combine the incredibly high specificities of other antibody-based protein purifications with an adequately good protein yield.
Name Catalog # Type Species Applications Size Price HighSpec Compact Cartridge Rho1D4 33302-CUBE Protein Purification 1 ml $768.00 HighSpec Compact Cartridge Rho1D4 33306-CUBE Protein Purification 5 ml $2,176.00 HighSpec Rho1D4 Agarose 33101-CUBE Protein Purification 1 ml $672.00 HighSpec Rho1D4 Agarose 33102-CUBE Protein Purification 5 ml $2,233.00 HighSpec Rho1D4 Agarose 33103-CUBE Protein Purification 10 ml $3,793.00 Highspec Rho1D4 Agarose XL 57603-CUBE Protein Purification 10 ml $4,515.00 Highspec Rho1D4 Agarose XL 57601-CUBE Protein Purification 1 ml $805.00 HighSpec Rho1D4 Antibody 40020-CUBE Protein Purification 200 ug $811.00 HighSpec Rho1D4 MagBeads 33201-CUBE Protein Purification 1 ml $740.00 HighSpec Rho1D4 MagBeads 33205-CUBE Protein Purification 5 ml $2,466.00 HighSpec Rho1D4 MagBeads XL 57705-CUBE Protein Purification 5 ml $3,024.00 HighSpec Rho1D4 MagBeads XL 57701-CUBE Protein Purification 1 ml $915.00 HighSpec Rho1D4 Peptide 16205-CUBE Peptide 100 mg $3,141.00 HighSpec Rho1D4 Peptide 16201-CUBE Peptide 5 mg $315.00 Rho Starter Set 1 33199-CUBE Protein Purification 1 Set $532.00 Rho Starter Set 2 33299-CUBE Protein Purification 1 Set $565.00 Rho Starter Set 3 33398-CUBE Protein Purification 1 Set $625.00 - GST-Tag Affinity Resins & MagBeads
The GST-tag is a 26 kDA sized protein tag that can be used for protein purification. GST is short for Glutathione-S-Transferase. Using the GST-tag for protein purification is taking advantage of an enzyme's specificity to a substrate to gain maximum purity.
Name Catalog # Type Species Applications Size Price PureCube Glutathione Agarose 32103-CUBE Protein Purification 10 ml $399.00 PureCube Glutathione Agarose 32112-CUBE Protein Purification 500 ml $12,122.00 PureCube Glutathione MagBeads 32205-CUBE Protein Purification 5 ml $597.00 - Strep-Tactin® Affinity Resins & MagBeads
The Strep-Tactin® purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin®, to Strep-Tactin® II fusion proteins. Cube Biotech offers a high-quality affinity resin for strep-tagged proteins, with maximum binding capacity. Choose your preferred method from either agarose resins or magnetic beads.
Name Catalog # Type Species Applications Size Price PureCube Compact Cartridge HiCap StrepTactin 34302-CUBE Protein Purification 1 ml $295.00 PureCube Compact Cartridge HiCap StrepTactin 34306-CUBE Protein Purification 5 ml $857.00 PureCube HiCap StrepTactin Agarose 34101-CUBE Protein Purification 1 ml $211.00 PureCube HiCap StrepTactin Agarose 34103-CUBE Protein Purification 10 ml $1,346.00 PureCube HiCap StrepTactin Agarose 34105-CUBE Protein Purification 50 ml $5,758.00 PureCube HiCap StrepTactin MagBeads 34201-CUBE Protein Purification 1 ml $239.00 PureCube HiCap StrepTactin MagBeads 34205-CUBE Protein Purification 5 ml $1,029.00 PureCube HiCap StrepTactin MagBeads 34225-CUBE Protein Purification 25 ml $3,851.00 PureCube HiCap StrepTactin MagBeads XL 57405-CUBE Protein Purification 5 ml $1,240.00 - Activated Resins & MagBeads
Activated agarose resins and MagBeads are off-the-shelf items for specialized purposes. They consist of our high-quality PureCube Agarose and MagBeads with active chemical groups on the surface.
Amine Activated
Amine Activated Agarose Resin and Magnetic Beads serve a specialized role in the laboratory. They are suitable to couple nearly any biomolecule to beads that carry a free Carboxy-Group which is able to form a bond with the active amino groups.
Name Catalog # Type Species Applications Size Price PureCube Amine Activated Agarose 51003-CUBE Protein Purification 10 ml $351.00 PureCube Amine Activated MagBeads 50905-CUBE Protein Purification 5 ml $1,477.00 Carboxy Activated
Carboxy Activated Beads carry a free carboxyl group that can be activated by adding N-hydroxy-succinimide (NHS). Proteins can then bind covalently via amine groups, forming a stable peptide bond.
Name Catalog # Type Species Applications Size Price PureCube Carboxy Agarose 50105-CUBE Protein Purification 50 ml $1,118.00 PureCube Carboxy MagBeads 50201-CUBE Protein Purification 1 ml $297.00 PureCube Carboxy MagBeads 50205-CUBE Protein Purification 5 ml $1,118.00 PureCube Carboxy MagBeads 50225-CUBE Protein Purification 25 ml $4,755.00 PureCube Carboxy MagBeads XL 58201-CUBE Protein Purification 1 ml $389.00 Epoxy Activated
Epoxy Activated Agarose resins and MagBeads are carrying an epoxy functionality. With this activated epoxy group proteins can be bound to the beads covalently via free amines, thiol groups, or hydroxyl groups.
Name Catalog # Type Species Applications Size Price PureCube Epoxy Activated Agarose 50705-CUBE Protein Purification 50 ml $1,337.00 PureCube Epoxy Activated MagBeads 50805-CUBE Protein Purification 5 ml $1,477.00 PureCube Epoxy Activated MagBeads 50825-CUBE Protein Purification 25 ml $5,492.00 Maleimide Activated
Maleimide Activated Agarose Resins and MagBeads are carrying an Maleimide functionality. With this activated maleimide, groups can be bound to the beads covalently via their thiol groups.
Name Catalog # Type Species Applications Size Price PureCube Maleimide Activated Agarose 51103-CUBE Protein Purification 10 ml $1,023.00 PureCube Maleimide Activated Agarose XL 59103-CUBE Protein Purification 10 ml $1,335.00 PureCube Maleimide Activated MagBeads 51201-CUBE Protein Purification 1 ml $800.00 PureCube Maleimide Activated MagBeads 51205-CUBE Protein Purification 5 ml $1,940.00 NHS Activated
PureCube NHS Activated Agarose carries a N-hydroxy-succinimide (NHS) functionality. Proteins can bind covalently via amine groups, forming a peptide bond.
Name Catalog # Type Species Applications Size Price PureCube NHS Activated Agarose 50303-CUBE Protein Purification 10 ml $677.00 PureCube NHS Activated MagBeads 50405-CUBE Protein Purification 5 ml $1,671.00 PureCube NHS Activated MagBeads 50425-CUBE Protein Purification 25 ml $6,228.00 - Alprenolol
Alprenolol agarose is a good example of ligand-based purification. It binds to the beta 1 and beta 2 adrenergic receptors and the ligand was coupled via a hydrophilic linker to highly crosslinked 6% agarose to minimize non-specific binding.
Name Catalog # Type Species Applications Size Price PureCube Alprenolol Agarose 56031-CUBE Protein Purification 1 ml $2,180.00