Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media.
Thus, if proteins from biotin containing extracts or media are intended to be purified via Strep-Tactin® chromatography, biotin must be masked by the addition of avidin prior to the application onto the column. BioLock biotin blocking solution allows this masking of biotin (and biotinylated proteins) in a very fast and convenient way.
Add at least 1U of BioLock solution per µg of biotin.
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Name Catalog # Size Biotin Blocking Buffer 2-0501-002 2 ml Strep-Tactin AP Conjugate 2-1503-001 0.5 ml Strep-Tactin HRP Conjugate 2-1502-001 0.5 ml Strep-Tactin® coated microplates 2-1501-001 1X96 Well Plate Strep-Tactin® coated microplates 2-1501-050 5X96 Well Plates StrepMAB-Classic 2-1507-001 100 ug StrepMAB-Classic HRP conjugate 2-1509-001 75 ul StrepMAB-Immo 2-1517-001 100 ug