When researchers employ our human primary cancer associated fibroblasts (CAFs) in their tumor microenvironment models, they consistently find stark differences between 2D and 3D. Through 3D co-culture, investigators recognize that their models mirror in vivo tumors more closely than in 2D.
This makes our CAFs invaluable, enabling drug discovery research that better mimics the tumor microenvironment. Furthermore, over the last few years, we've seen the observation across many of our CAF types, including pancreatic, colorectal, breast, and now, ovarian.
Image: Human ovarian cancer cell lines (SKOV3 and OVACR8) along with Neuromics ovarian CAFs (cat. CAF112) in 2D and 3D ovarian cancer models.
Earlier this month, researchers from Taiwan utilized our Ovarian CAFs (cat. CAF112) to create a 3D ovarian cancer tumor microenvironment model. By integrating CAFs in their 3D model, the researchers found that it better exemplified the in vivo tumor microenvironment than 2D culture or models without CAFs.
Increased tumorigenicity and chemoresistance were observed in the 3D sphere model with CAFs compared to 2D co-culture models. These results help validate their model as a good representation of the tumor microenvironment and one of value to research therapeutic approaches. You can read the full paper here.
Bottom image: Formalin-fixed paraffin-embedded tumor sections stained with various markers with or without integration of ovarian cancer spheres with or without CAFs.
Neuromics offers a wide selection of CAFs, with nearly a dozen types of normal CAFs. Furthermore, we have hTERT immortalized many types, making those options also available. Check them all out below.
| Name | Catalog # | Type | Species | Applications | Size | Price |
|---|---|---|---|---|---|---|
| Human Breast CAFs | CAF116 | Human Primary CAFs | Human | Cell Assays | 1,000,000 cells | $2,792.88 |
| Human Bronchial CAFs | CAF111 | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $3,078.00 |
| Human Colorectal Tumor CAFs | CAF115 | Human Primary CAFs | Human | Cell Assays | 1,000,000 cells | $1,294.92 |
| Human Kidney CAFs | CAF110 | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $3,078.00 |
| Human Liver CAFs | CAF113 | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $3,078.00 |
| Human Lung Adenocarcinoma CAFs | CAF117 | Human Primary CAFs | Human | Cell Assays | 1,000,000 cells | $1,294.92 |
| Human Lung Squamous Cell Carcinoma CAFs | CAF117S | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $3,078.00 |
| Human Metastatic Ovarian CAFs | CAF114 | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $3,078.00 |
| Human Ovarian CAFs | CAF112 | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $3,078.00 |
| Human Pancreatic CAF-Stellate Cells | CAF118 | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $2,656.80 |
| Human Prostate CAFs | CAF119 | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $3,078.00 |
| Immortalized Human Colorectal Tumor CAFs | CAF115-IM | Human Primary CAFs | Human | Cell Assays | 1,000,000 cells | $7,018.92 |
| Immortalized Human Kidney CAFs | CAF110-IM | Human Primary CAFs | Human | Cell Assays | 1,000,000 cells | $9,536.40 |
| Immortalized Human Lung Adenocarcinoma CAFs | CAF117-IM | Human Primary CAFs | Human | Cell Assays | 1,000,000 cells | $7,018.92 |
| Immortalized Human Lung Squamous Cell Carcinoma CAFs | CAF117S-IM | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $9,536.40 |
| Immortalized Human Ovarian CAFs | CAF112-IM | Human Primary CAFs | Human | Cell Assays | 1,000,000 Cells | $9,536.40 |
| Immortalized Human Pancreatic CAFs | CAF118-IM | Human Primary CAFs | Human | Cell Assays | 1,000,000 cells | $8,596.80 |