Potent, Proven, and Ready to Use 3-D Models
Neuromics offers Primary Human Cell Based Blood-Brain Barrier (BBB) and Human Umbilical Vein Endothelial Cell (HUVEC) Models.
These models are designed to study specific diseases. There are configured to mimic in-vivo like environments. We provide the cultured cells plates with 3-D inserts. The 3-D environments are optimized for cell growth in 3-D and ready to use for your drug discovery and toxicology assays.
Analytes tested by our customers include small molecules. compounds, proteins, antibodies, viruses, and bacteria.
Custom Models
Customers also use Neuro, Cancer-Associated Fibroblasts (CAFs), Cancer and iPSC-Derived Cells to manufacture organic, and micro-fluidic models.
Penetration study in 3D Human BBB Model
Organoids-Induction, Growth and Maintenance
Organoids are highly valuable because they replicate the structure and function of human organs on a miniature scale, enabling precise research into disease, drug responses, and personalized medicine.
Neuromics' Organoid Media are chemically defined, serum-free, animal component–free media optimized and formulated to support the self-organization of endothelial cells, pericytes, and mesenchymal cells into complex 3D structures that recapitulate the architecture and function of human blood vessels, including capillary networks and arteriole-like structures.
Vascular Organoid Media
Phase | Days | Medium |
Mesoderm Induction | 0–6 | |
Vasculogenesis | 6–18 | |
Maturation | 18–35+ |
Primary Human Astrocyte and Human Umbilical Cell (HUVES) Organoids
Yvonne Adams, Anja T.R. Jensen (University of Copenhagen) manufactured Organioids using Neuromics' Primary Human Astrocytes and Human Brain Microvascular Endothelial Cells to study Plasmodium falciparum erythrocyte membrane protein 1 variants induce cell swelling and disrupt the blood–brain barrier in cerebral malaria.
Cerebral malaria (CM) is caused by the binding of Plasmodium falciparum–infected erythrocytes (IEs) to the brain microvasculature, leading to inflammation, vessel occlusion, and cerebral swelling.
Image 1: 3D BBB spheroids are composed of three different cell types. (A) FACS histogram showing pericytes (NG2, neural/glial antigen 2), astrocytes (GFAP, glial fibrillary acidic protein), and human cerebral microvascular endothelial cells (hCMEC/D3 and CD31).
Image2: ICAM-1–enriched microvilli and transmigratory ring/docking structures on brain hCMEC/D3 endothelial cells. (A–C) hCMEC/D3 brain endothelial cells were incubated with parasites.
This is first time the presence of intact, mature P. falciparum IEs within brain microvascular endothelial cells both in vitro and in vivo. The data on the enhanced binding and internalization of IEs suggest that the same approach will be necessary to counteract not only the effects of cytoadhesion but also the subsequent contribution to potentially lethal brain swelling in CM.
More on 3-D "in-vivo" Like HuCell Models
More Blood-Brain Barrier Research
We are always excited to showcase innovative publications utilizing our reagents. We take immense pride in providing researchers and professionals with reliable and consistent products that unlock new possibilities in their fields. Today, we delve into another new study using some of of our primary human brain cells.
Researchers from the University of Nottingham have released two new publications using our primary human brain microvascular endothelial cells (cat. HEC02), brain pericytes (cat. HMP104), and brain astrocytes (cat. HMP202) to build an in vitro blood-brain barrier (BBB) model. Last week, we highlighted the first publication, where the impact of exposure to Substance P (SP) on the BBB was evaluated. You can check out the blog post to learn more.
Image: Astrocytes stained with S100 beta.
Then, to our pleasant surprise, we saw the researchers cited our cells in another BBB related publication. This time, the investigators looked into some of the mechanisms behind neuroinflammation seen in ischaemic stroke and other disorders. They found that inhibition of Rho kinase neutralized the disruptive effects of TNF-α on BBB integrity. You can read the full study here.
Along with our human cells, we have the culturing tools to keep them happy and healthy throughout the entirety of your experiment. This includes fetal bovine serum (FBS), cell-specific growth media, coating solutions, detachment solutions, and more. A customer just mentioned to us that our endothelial cells demonstrate "increased resistance and avoid both senescence and apoptosis induced by oxysterols" when using our ENDO-Growth Media (cat. MED001) in comparison to competitor formulations.
Blood-Brain Barrier Publication
As always, Neuromics greatly appreciates when our products are used in publications. The most recent publication shared by customers took advantage of our mouse Tuj-1 antibody (Cat.#MO15013). In this publication, researchers describe how they created an ex vivo 3D vascularized neural constructs that mimics the function of the blood-brain barrier.
Image: The neurovascular interface containing Tuj1+ (green) neurons differentiated from NSCs (in spaces surrounding the vasculature network) and Claudin-5+ (red) endothelial cells (inside the microfluidic channels). Scale bar, 500 μm. Image citation below
Citation:
Haibing Yue, Kai Xie, Xianglin Ji, Bingzhe Xu, Chong Wang, and Peng Shi. (2020). Vascularized Neural Constructs for Ex-Vivo Reconstitution of Blood-Brain Barrier Function. Biomaterials. doi: 10.1016/j.biomaterials.2020.119980
Learn more...
In addition to our Tuj-1 antibody used in this publication, Neuromics offers a wide selection of antibodies ideal for BBB research. Check them out here!
Image: Spatial distribution of neurons (Tuj1+, green) in the 3D neural construct mimicking the in vivo BBB. The gray dash lines indicated the interfaces of collagen and tubal networks. Citation same at the above image
Our Reagents are Helping Answer Questions About COVID-19
With COVID-19 affecting the globe for well over a year at this point, many researchers have shifted their focus to the virus. While there are many COVID-19 related questions still needing answers, it is incredible what the scientific community has achieved in such a short period of time.
Our reagents have been used by customers in a handful of publications that have helped us learn more about the virus. We've just added a new page to our website to compile all these publications. Visit it here.
Image: Detected auto Ig levels in specific cell types, including our human small intestine endothelial cells.
Included is a publication from researchers at Emory University comparing the plasma of COVID-19 patients to healthy patients to study the pathogenesis of severe COVID-19. In this research, they used our Human Small Intestine Endothelial Cells (cat.# HEC15). Check out this blog post to learn more.
A second publication utilizes our Human Brain Microvascular Endothelial Cells (cat.# HEC02) to find a microRNA capable of targeting Neuropilin-1, a transmembrane glycoprotein that plays a role in the cellular entry of the SARs-CoV-2 virus. Learn more.
Neuromics' continues to grow our selection of products that have applications in COVID-19 research. In case you missed it, we just added six recombinantly expressed MHC class I molecules that present a peptide from the SARS-CoV-2 virus. Additionally, we recently generated data demonstrating our 3D Human Blood-Brain Barrier Model (cat.# 3D45002) expresses the ACE-2 receptor, the protein that the COVID-19 virus binds to.
You can view all of our COVID-19 related research reagents here.