Neuromics FBS + Health Research
Scientists from Augusta University used our Premium Imported Fetal Bovine Serum (FBS) (cat. FBS002) to culture RAW264.7 cells, a mouse macrophage line, in preliminary research released this month. They were looking at ways to deliver nutraceuticals in oral capsules or beverages that allow the body to better process the nutrients.
Image: RAW264.7 cells culture using Neuromics Fetal Bovine Serum (FBS).
The study presents the Facilitated Self-Assembling Technology (FAST) platform, a clean-label method for generating bioavailable nutraceutical nanoparticles. Many other platforms use harmful compounds or are not able to keep nutraceutical compounds stable.
Researchers Prefer Our Preferred FBS
Chronic pain is a condition that affects tens of millions of Americans. Pain management has generally relied on NSAIDS and opioids, both of which have drawbacks. Put simply, novel pain therapeutics are needed.
Collaborating researchers from the University of New Mexico released a study earlier this month about an experimental chronic pain therapeutic. They relied on our Preferred Fetal Bovine Serum (cat. FBS007) for the cell culturing in their study.
Image: Immunostaining of human DRG cultured using Preferred FBS.
The scientists decided to target the P2X4 receptor (P2X4R), known for its role in pain signaling, with a humanized single-chain variable fragment (hscFv) targeting P2X4R. hscFvs are genetically engineered antibody fragments that are starting to become of increased interest in drug discovery.
After culturing human DRG neurons, HEK-293T cells, and HEK-293T cells stably expressing human His-tagged P2X4R with our FBS, the samples were pretreated with the hscFv targeting P2X4R. They found that P2X4R activity and neuronal excitability were both decreased in cells treated with the hscFv compared to the control.
Breast CAFs & FBS Collaborating Harmoniously
Far and away, the most common research theme with our human cancer associated fibroblasts (CAFs) is 3D. Whether it's 3D co-culture, organoids, or in vivo models, the value of these cells is most apparent when integrated into 3D models that mimic the tumor microenvironment (TME). A new study from scientists at the Federal University of Santa Catarina in Brazil outlines a novel approach for building a 3D platform for co-culturing cells. The investigators utilized our human primary breast CAFs (cat. CAF116) and US origin premium fetal bovine serum (FBS) (cat. FBS001) to make it happen.
Image: 3D confocal microscopy of cells seeded into BNC at different time points: MDA-MB-231 cells (green), Neuromics breast CAF (red), and M2 macrophages (blue). MDA-MB-231 cells and macrophages were grown with Neuromics’ FBS.
The investigators outlined the steps to build a translucent multi-compartmentalized stacked multilayered nanocellulose scaffold. The scaffold consisted of bacterial nanocellulose (BNC) layers separated by interlayers of a lower density of nanocellulose fibers. To validate their scaffold model, they cultured several cell types, including EOMA cells, EA.hy926 cells, MDA-MB-231 cells, monocytes, and macrophages with media supplemented with Neuromics' FBS.
Culture Any Cell Type with Our FBS
Over the years, Neuromics fetal bovine serum (FBS) has been utilized in numerous research papers and projects. One of the main takeaways from the papers is the breadth of cell types and applications that our FBS options enable.
Whether you're working with sensitive primary cells, robust cancer cell lines, stem cells, or anything in between, we undoubtedly have a solution. Furthermore, if your application calls for more than basic FBS, we supply heat-inactivated and specialty options allowing you to satisfy your specific needs.
That trend continues, with a new thesis released this week. In the paper, a student from the University of Arkansas used our heat-inactivated premium imported FBS (cat. FBS002-HI) to culture RTS11 cells, a rainbow trout monocytic cell line, and monocytic cells isolated from rainbow trout kidneys. The research explored how temperature impacts immune function. Learn more here.
Cancer & Pain Intersect with Our Schwann Cells
Neuroscience, cancer, and pain - these are the three areas where Neuromics products are most prevalent. Last month, researchers from New York University published a study at the intersection of these three fields, using a neural cell type to explore the mechanisms of pain in oral cancer.
Image: TRPV4 is functionally expressed in Neuromics human Schwann cells. Expression is confirmed using TRPV4 agonist GSK1016790A.
Oral cancer is known to often cause a lot of pain when the lesion is touched or pressured, but the cause of this pain is not well understood. When looking at some of the mechanisms of this pain, the scientists employed our primary human Schwann cells (cat. HMP303). The investigation focused on the expression of TRPV4 in Schwann cells, including our Schwann cells. They found that TRPV4 inhibition reduced pain in mouse models, suggesting TRPV4 from activated Schwann cells is a major source of pain in oral cancer. You can read the full study here.
