Human Primary Cells, iPSC Derived Cells & Cell Lines Continued...

With colorectal cancer (CRC) being one of the most prevalent cancers worldwide, understanding chemotherapy resistance in the CRC tumor microenvironment (TME) is an important goal. As we’ve seen in many other publications using our human cancer-associated fibroblasts (CAFs), CAFs play a huge role in the TME.

Our CAFs have been used in a handful of studies in 2D and 3D TME models, where they demonstrate their ability to complicate treatment objectives. Overall, the mechanisms by which CAFs contribute to chemotherapy resistance in the TME are poorly understood. 

Image: DKK1 expression in Neuromics colorectal CAFs when subjected to chemotherapeutic agents.

Researchers from the University of Kentucky utilized our colorectal CAFs (cat. CAF115) in a new paper examining these mechanisms. In their cell models, they found that CAFs secrete DKK1, a protein associated with tumor growth and metastasis, when subjected to chemotherapy treatments for CRC. Their results identified CAF-secreted DKK1 as a major player in resistance to treatment. Furthermore, they recommend looking into treatments that target DKK1 to counteract chemotherapy resistance.

You can check out the full paper here.

Neuromics specialize in providing reagents and methods for the Neuroscience, Immunology, Cancer, Stem Cell and Regenerative Medicine Research Community.  We serve scientists doing basic research and drug discovery in Neurodegenerative Diseases, Pain, Autoimmune Diseases and Cancer. Our unique value is based on providing reagents that are thoroughly tested and characterized.

You are doing some amazing research. I'm excited to share with you a small sampling of the interesting research being done with Neuromics solutions. 

First I'd like to share a recent publication using our ABCA1 Antibody: Jianjia Fan, Rui Qi Zhao, Cameron Parro, Wenchen Zhao, Hsien-Ya Chou, Jerome Robert, Tarek Z. Deeb, Carina Raynoschek, Samantha Barichievy, Ola Engvist, Marcello Maresca, Ryan Hicks, Johan Meuller, Stephen J. Moss, Nicholas J. Brandon, Michael W. Wood, Iva Kulic, Cheryl L. Wellington. (2018). Small molecule inducers of ABCA1 and apoE that act through indirect activation of the LXR pathway. Journal of Lipid Research. doi: 10.1194/jlr.M081851

With fall research already starting or quickly approaching, there is no better time to stock up on the reagents needed to enable discoveries. Fortunately, Neuromics has your back. In addition to our human cells, FBS, and media & culturing tools, among others, we've just added over 40 new antibodies.

Image: Immunofluorescent analysis of a section of adult rat hippocampus stained with a new goat pAb to Fox3/NeuN, GT22106, dilution 1:2,000 in red, costained with mouse monoclonal antibody to MAP2 in green. Nuclear DNA was revealed in blue using the DAPI stain.

These antibodies are perfect for researchers across a broad spectrum of specialties. There are antibodies for neuroscientists, including markers for CNS cells and neurological disease, including TBI and neurodegenerative diseases. Furthermore, these new antibodies cover cancer research, cardiovascular research, and so much more. Read more to see for yourself.

In addition to these new antibodies, Neuromics has an existing selection of research proven antibodies with a wide variety of applications. Explore them here. Many of these antibodies are currently on sale and 20% off! Check out the on sale antibodies here.

In dorsal root ganglion (DRG) neurons, ATP is an important neurotransmitter in nociceptive signaling through P2 receptors (P2Rs) such as P2X2/3R, and adenosine is also involved in anti-nociceptive signaling through adenosine A1R.

Check out this publications using our P2X2 Receptor (GP14106) Antibody, where ENNPs interact with P2XRs to metabolize ATP to AMP in DRGs. 

Image: Rat DRG stained with Neuromics P2X2R and ENPP1.

Our mission to Mars is driving a need to better understand the physiological impact on the human body. Deep space travel places unique challenges for humans. It is important that there is minimal impact on space travelers' senses. This includes hearing. This study uses our Shank 1a Antibody to determine changes in the neuronal structure of the ear in microgravity.

Top Image: Verification of antibodies to CtBP2 and Shank1a. A and B: serial 14-µm cryosections were obtained from a postnatal day 71 (P71) mouse utricle, and maximum-intensity projections are shown. Hair cell (hc) and support cell (sc) nuclei are illuminated by the DAPI stain (blue). Numerous closely associated CtBP2-and Shank1a-positive puncta can be observed in the positive immunostained section represented in A (block arrowheads). The CtBP2-positive puncta highlighted by the flared arrowhead in A may represent an undocked synaptic ribbon. No primary antibodies were included in the processing represented in the micrograph in B. The scale bar in B represents 5 µm and also applies to A. C and D: maximum-intensity projection micrographs from right and left whole mount utricles from a P65 mouse...

Having problems with bad backgrounds? Does this problem compromise your data?

Worry no more, because we have a solution...

FluoMute^TM 

FluoMute™ ready-to-use reagent reduces autofluorescence in cells and tissue. Just incubate fixed cells of tissue sections with FluoMute™ for 30-60 min at room temperature, rinse with PBS and continue with immunofluorescence ICC or/and IHC protocols.

Tyrosine hydroxylase TH is a necessary enzyme to create neurotransmitters and protect the body against oxidative stress.

Dysregulation of this enzyme results in manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.

Image: TH staining of mouse salivary gland tissue. https://doi.org/10.1016/j.omtm.2018.02.008

Neuromics is excited to tell you about new antibodies to our catalog. 

Image: Immunofluorescent analysis of (A) C6 rat glioma cells and (B) Human embryonic kidney cells stained with mouse mAb to fibrillarin, MO22169, dilution 1:1,000 in red, in both cases costained with chicken pAb to vimentin, CH22108, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA.

Neuromics is pleased to add a wide selection of antibodies perfect for many types of research. In particular, we've added Ankyrin 3/Ankyrin G antibodies, FABP7 (Fatty Acid-Binding Protein 7) antibodies, and many more.

Image: Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with mouse antibody to GFP, MO22184, dilution 1:1,000 in red. The blue is Hoechst staining of nuclear DNA. The MO22184 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.

Check out the new antibodies below...

It's always great to hear from our clients and see the data that is being generated using our products. The following are examples of data being generated from people like you using Neuromics' products. 

Rat DRGs stained with VR1 antibody (cat# RA10110). The tissue was perfused, cryoprotected in 30% Sucrose-PBS and frozen sectioned to slides at 10um.  The tissue was labeled with VR1 (green) at 1:1000 and NeuN (red) at 1:1000.  This pictures was taken with an epifluorescent microscope at 10x. Thanks to Joanne Steinauer at University of California, San Diego for sharing

Time is money. This is especially true of routine immunostaining assays.

Thus, we are pleased to introduce you to EZ-HRP™ Polymer Detection. EZ-HRP™ Polymer Detection's features allow its users to save time, and as a result, money.

Image: Comparison of IHC images using EZ-HRP™ Polymer vs. a conventional avidin-biotin antibody. 

We continue to add new antibodies. Many are additions to our frequently used and widely published Neuron-Glial Markers. All our products are tested, characterized and research ready.

Image: Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43, MO22170, dilution 1:1,000, in red, and costained with chicken pAb to MAP2, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, while the MAP2 antibody stains dendrites and perikarya of neurons.

Check out our new antibodies...

Neuromics is pleased to offer a selection of Calbindin-1 antibodies that are perfect for reserach across a wide variety of areas. These research proven antibodies are some of our most popular antibodies in part because of their flexibility. Our Callbindin antibodies can be used for western blotting, immunocytochemistry, immunohistochemistry, and immunofluorescence.

Image: Adult mouse cortex (L) and cerebellum (R) sections were stained with MO22163 in red. Calbindin is expressed in a subset of interneurons in the cortex (L) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (R).

Learn more about these antibodies...

As always, Neuromics greatly appreciates when our products are used in publications. The most recent publication shared by customers took advantage of our mouse Tuj-1 antibody (Cat.#MO15013). In this publication, researchers describe how they created an ex vivo 3D vascularized neural constructs that mimics the function of the blood-brain barrier.

Image: The neurovascular interface containing Tuj1+ (green) neurons differentiated from NSCs (in spaces surrounding the vasculature network) and Claudin-5+ (red) endothelial cells (inside the microfluidic channels). Scale bar, 500 μm. Image citation below

Citation:

Haibing Yue, Kai Xie, Xianglin Ji, Bingzhe Xu, Chong Wang, and Peng Shi. (2020). Vascularized Neural Constructs for Ex-Vivo Reconstitution of Blood-Brain Barrier Function. Biomaterials. doi: 10.1016/j.biomaterials.2020.119980

Learn more...

The blood-brain barrier has become a focal point of drug discovery and toxicology research. Understanding the complicated mechanisms of the blood-brain could lead to many new treatments and therapies. Neuromics offers a selection of research proven antibodies perfect for BBB research. 

Image: Mixed neuron-glial cultures stained with Mouse Monoclonal GFAP (MO22136), and Chicken Polyclonal Neurofilament-NF-L(green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA. This antibody also works on formalin fixed paraffin embedded brain tissues. Protocol on Datasheet.

Linked are customer publications using our GFAP and Nestin

Check out these antibodies...

Cluster of Differentiation proteins are a collection of cell surface molecules with many members. Cluster of Differentiation proteins have been shown to be pivotal to both cancer research and immune response research. Now, if you're looking to use CD antibodies in your research, it's your lucky day! Neuromics has just added 13 CD antibodies to the website.

Image: Immunohistochemistry staining of MO47044 on human tonsil tissue.

To access the complete list of CD antibodies offered by Neuromics, click the link. 

Check out the new antibodies below...

When Neuromics opened in 2005, we solely provided neuroscience research antibodies. While we have expanded our selection products beyond neuroscience research antibodies, these antibodies remains at the core of our business. That's why it's still satisfying to see publications from customers using these antibodies (view them all here), like in three neuroscience publications new this month we'd like to highlight.

In the first publication, Iwate Medical University scientists studied the morphology of P2X3 in rat models utilizing our rabbit anti-P2X3 antibody (cat.# RA10109). Specifically, the investigators explored how P2X3-immunoreactive nerve endings and serosal ganglia interact in the rat gastric antrum.

Image: P2X3 (RA10109) Staining in Rat DRG. Working dilution 1:100 - 1:300.

Read more to learn about the other two publications...

Already this year, our Chicken Tuj-1 (Neuron-specific Class III beta-Tubulin) antibody (Cat.# CH23005) has been used in two publications, demonstrating the versatility and reliability of this antibody. This trend isn't new, as our Tuj-1 antibody has always been one of our most popular and research proven antibodies (here's our proof). Today, Neuromics would like to highlight its use in neurodegenerative disease and neurodevelopment research. However, no matter what type of neuroscience research you're engaged in, we encourage you to check it out!

Earlier this month, in the first publication we'd like to highlight, researchers from the University of British Columbia released their neurodegenerative disease research while utilizing our Chicken Tuj-1 antibody. Their research took advantage of a recently developed mouse model bearing a nonsense mutation at arginine 493 (R493X). This mutation has been shown to play a role in development of frontotemporal lobar degeneration (FTLD), which causes a clinical presentation of frontotemporal dementia.

Image: Representative hippocampal/thalamic tilescans highlighting the neuronal (TUJ1, green) TDP-43 (red) proteinopathy phenotype in the ventral thalamus of 18 month old GrnR493X/R493X mice (scale bar, 500 µm).

Read more to learn about their findings and the second publication...

We have research proven antibodies with uses across a wide variety of research areas. Our PGP 9.5 antibody (Cat.# GP14104) exemplifies this, being used in two studies that were published this past week highlighting the many fields in which our antibodies are applicable. Find all customer publications using our PGP 9.5 antibodies here.

The first study looked at how the immune system and neural system interact to cause abdominal pain. Specifically, they found that enteric glia play a role in connecting the two by disrupting the glial-mediated activation of macrophages. This suggests that enteric glia could be a focus of potential therapies of abdominal pain.

Top Image: Images showing immunolabeling in colon samples for M-CSF in human myenteric ganglia from individuals with Crohn disease along with control colon samples from patients that underwent resections for bowel trauma, volvulus, or intestinal bleeding.

Learn more...

We value feedback from our customers and take pride in seeing our products being used in meaningful research. Today, we'd like to highlight two recent publications using our GFAP antibodies in neurodegenerative disease research.

In the first publication, our rabbit GFAP antibody was used in a study that looked at how the DYRK1A inhibitor KVN93 regulates cognitive function, amyloid beta pathology, and neuroinflammation. Using a mouse model, they found that KVN93 reduced amyloid beta plaque levels, enhanced long term memory through better dendritic synaptic functions, and decreased neuroinflammation. These results suggest that KVN93 is a potential therapeutic drug for the treatment of Alzheimer’s disease and other neurodegenerative or neuroinflammation-related diseases.

Top Image: GFAP levels in the cortex and hippocampal CA1 (top) and DG regions (bottom) of 3-month-old 5x FAD mice injected with KVN93 or vehicle. KVN93 significantly decreases Aβ-mediated microglial and astrocyte activation in 3-month-old 5x FAD mice.

Learn more...

At Neuromics, we strive to continuously expand our catalog of antibodies to make sure researchers can find the reagents they need. We'd like to highlight several new antibodies that have been added in the past week that are great for research across a wide variety of areas. These new antibodies include markers for Mylein Basic Protein, PEA-15, Beta-Synuclein, and Annexin A5 which can be used to make great immunofluorescent or western blot images.

Image: Immunofluorescent analysis of rat cerebellum section stained with rabbit pAb to beta-synuclein, RA22141, dilution 1:1,000 in red, and costained with chicken pAb to parvalbumin dilution 1:5,000 in gree. The blue is Hoechst staining of nuclear DNA.

Learn more...

We often highlight the countless areas of study that our antibodies can help researchers. We encourage you to check out all our primary antibodies here along with the many applications they've been used for here.

Now, the three most prominent and common uses for our antibodies are in cancer, neuroscience, and pain research. Well, what do you know? Already this month, new research has been released utilizing our antibodies in those three corresponding areas. We'd like to highlight those studies below.

Image: Staining of various glioblastoma cell lines with Neuromics Humanin antibody (cat. RA19000).

In the first study, Korean researchers used our Humanin rabbit polyclonal antibody (cat. RA19000) to study glioblastoma progression. In their release of preliminary research, the investigators found humanin to encourage glioblastoma progression via the integrin alpha V (ITGAV)–TGF beta (TGF) signaling axis. Read the full publication here.

A couple months ago, we published a blog post highlighting the 20+ years of results our pain antibodies have demonstrated (learn more here). It didn't take long into 2023 to add on another year to the total. Earlier this month, researchers from the University of Texas Health Science Center at San Antonio published preliminary findings with the help of Neuromics' Guinea Pig VR1 C-Terminus/TRPV1 antibody (cat. GP14100).

Image: Immunofluorescent images of WGA and TRPV1 (cat. GP14100) staining of TG tissues in male and female mice with colocalization of TRPV1 and WGA indicated with arrows.

The researchers noted that orofacial pain is clinically understood to be more common in females than males. However, despite this understanding, no research had been done to look at what factors cause this occurrence. Using mice as a model, the investigators began to characterized the sex-specific differences in gene expression, biological pathways, and molecular function of tongue sensory neurons. Our antibody played a big part in their analysis. We encourage you to check out the full publication below to learn more about their findings.

Neuromics has been successfully providing pain research antibodies for 20+ years. They are widely used and frequently published.

Image: Mouse inferior olive stained with Neuromics guinea pig TRPV1 antibody (cat. GP14100) using Cy3-conjugated donkey anti-guinea pig secondary antibodies (red color) and DAPI (blue) as a nuclear counterstain.

Here's a sampling of our top sellers:

• Mu Opioid Receptor
• VR1 C-Terminus (TRPV1)
• Substance P
• Alpha 2a Adrenergic Receptor
• NPY Y2 Receptor
• ORL-1/Opioid Receptor Like 1
• TRPA1

When discussing our primary antibodies for pain and neuroscience research, we always point to the 20+ years of results. Why wouldn't we? That's quite an accomplishment. Several of these antibodies have been consistently used in published research for over two decades. Over the weekend, two more studies were released using our antibodies in these familiar applications.

Image: Increased expression of mu opioid receptor levels in the ipsilateral lumbar DRGs of CPSS rats.

Researchers from Johns Hopkins University released pain research with the help of our rabbit Mu Opioid Receptor antibody (cat. RA10104). The paper introduced a rat model to study chronic pain after spine surgery (CPSS). By performing surgery on the rats and looking for symptoms of CPSS after the procedure, it provides a model to study the mechanisms of the disorder. You can read the full paper here.

At the core of the Neuromics portfolio are reagents with applications in neuroscience research. As a result, we see a large portion of references relating to neurodegenerative diseases, the brain, pain, and other neuroscience fields.

And while our collection of customer publications proves that our products can be used for so much more (see for yourself here), that theme of neuroscience research continued this past week. Two publications were released using our research tools in Parkinson's disease (PD) studies that we'd like to highlight.

Image: GFAP staining (cat. RA22101) of various parts of the PD mouse brain.

In the first study, researchers from Korea used our Rabbit GFAP Polyclonal Antibody (cat. RA22101). They evaluated various antihistamines as potential therapies for patients with PD. After analyzing South Korean patient data and using various animal models, they found that fexofenadine is a potentially effective option to treat PD. Our antibody was used to stain various parts of the mouse brain in a PD model. We encourage you to check out the full publication here.

Over the past few weeks, we've seen a bunch of new publications come through from customers highlighting the capabilities of our many products. These include two studies using some of our highly touted pain research antibodies in migraine and neuropathic pain research. Additionally, one of our primary human cell types was utilized in pancreatic cancer research. You can explore all citations using Neuromics products here.

In the first piece of research, migraine researchers used two of our Neuropeptide Y (NPY) receptor antibodies. The investigators injected mice with glyceryl trinitrate (GTN) in the medial habenula (MHb) to produce a migraine mouse model. Upon microinjections of NPY, the signs of migraine decreased in the GTN mice. Using our Rabbit Polyclonal NPY Y1 Receptor Antibody (cat. RA24506) and Rabbit Polyclonal NPY Y2 Receptor Antibody (cat. RA14112), the scientist observed activation of NPY Y1 receptors, but not NPY Y2 receptors.

Image: Immunofluorescent staining showing the expression of the Y1 receptor (cat. RA24506) and Y2 receptor (cat. RA14112) in the MHb of GTN mice after microinjections of NPY.

We wanted to remind you about all of the research tools we have available for neuroscience researchers. From human cells to antibodies and more, we have plenty to choose from.

At the forefront of our neuroscience product offerings are our Primary Human Neurons (cat. HNC001). Pictured in culture to the right, these neurons are isolated from donor brain cortical tissue and are versatile in their applications.

For example, earlier this month, our neurons, along with our Human Brain Astrocytes (cat. HMP202), were both utilized in neuroblastoma research. The scientists were looking at T cells expressing chimeric antigen receptors (CARs) targeting glypican-2 (GPC2) as a possible clinical target for neuroblastoma and other solid tumor treatment. Using our cells, the researchers determined that mature neurons and astrocytes express low levels of GPC2, making them a safe target for therapy. In addition to our cells, the researchers also used media and other culturing products supplied by Neuromics.

Click here to check out the publication for yourself!

We encourage you to read more to learn about some of our other neuroscience related products...

In the past couple of weeks, our blog has shared studies using Neuromics GFP-expressing cells and cell lines in exciting research. Now, we are happy to highlight another new publication that merges the two—a GFP-expressing cell line. Last month, researchers at Dartmouth published a paper using our GFP-expressing human glioblastoma cells (U87 MG) (cat. TR01-GFP) in fluorescent-guided surgery research. 

Contrast imaging has become a frequently used tool in surgical resection so that surgeons can remove all tumor tissue when operating on patients. This method has especially become prevalent in glioma removal. While many methods for fluorescent-guidance surgery exist, the investigators wanted to look for one that accurately marks tumors shortly after administration and is compatible with current imaging capabilities.

Image: Imaging of mice inoculated with GFP expressing U87 MG cells after 40 and 90 minutes. A comparison with GFP shows how candidate fluorescent agents perform.

When researchers employ our human primary cancer associated fibroblasts (CAFs) in their tumor microenvironment models, they consistently find stark differences between 2D and 3D. Through 3D co-culture, investigators recognize that their models mirror in vivo tumors more closely than in 2D.

This makes our CAFs invaluable, enabling drug discovery research that better mimics the tumor microenvironment. Furthermore, over the last few years, we've seen the observation across many of our CAF types, including pancreatic, colorectal, breast, and now, ovarian.

Image: Human ovarian cancer cell lines (SKOV3 and OVACR8) along with Neuromics ovarian CAFs (cat. CAF112) in 2D and 3D ovarian cancer models.

There is a well choreographed dance between astrocytes, glia. microglia and neurons in the brain. This dance in is highly compartmentalized among different brain cell types to efficiently support various energy-demanding processes. 

Astrocytes play a special role. They regulate synaptic transmission fine tuning metabolism and energy management. Another role is supply metabolites such as lactate, serine, and glutamine to support neuronal activity and supply energy. 

Here, Neuroscientists, published in "Nature Communications." uncover the role of an endolysosomal transmembrane protein in mediating catabolic processes in astrocytes. Researchers used Neuromics' Primary Human Brain Astrocytes for this study.

Top Image: Graphical representation of study conclusions.

Autophagic flux may work in tandem with reduced LD catabolism to limit the mobilization of toxic lipid species that cause inflammatory cascades in the brain. The neuron-astrocyte. Better understanding flux could be of value in new research approaches for understanding the roots of Neurodegenerative Diseases

Neuromics continues to expand our selection of immortalized human cells. This week, we've added eight new types of immortalized primary cells. You can passage these immortalized cells over 20 times, giving you more bank for your buck.

Top image: Neuromics primary human umbilical vein endothelial cells (cat. HEC01).

These newly released cells are fibroblasts and endothelial cells. Fibroblasts are one of the most widely used types of primary cells, as they are easy to culture and reprogram. Likewise, endothelial cells offer a great model to study vascular biology, angiogenesis, and inflammation.

Read more to check out the new cells.

Just how important is the tumor microenvironment (TME) in cancer research? As a supplier of primary human cancer-associated fibroblasts (CAFs), we’re exposed to plenty of studies looking into their role. One big theme: it’s becoming ever clearer that cancer treatments must take CAFs and their role in the TME into account.

Image: 3D organotypic colorectal tumor model surrounded by CAFs (vimentin, green) (a). Organoids are larger and more plentiful when CAFs are incorporated than without CAFs (b & c).

In a newly released paper, a team of researchers from the University of Akron builds 3D organoids with patient-derived colorectal tumor samples to study prospective cancer treatments. They compare how organoids develop, prosper, and react to treatments when they are with and without colorectal tumor CAFs (cat. CAF115) provided by Neuromics.

Late in 2024, Dartmouth researchers published a paper using our GFP-expressing human glioblastoma cells (U87 MG) (cat. TR01-GFP) in fluorescent-guided surgery research. The scientists cultured Neuromics GFP-expressing U87 MG cells and then inserted them into a mouse model. After testing several fluorescent agents, they found one, tetramethylrhodamine conjugated to a small polyethylene glycol chain (TMR-PEG1k), performed the best (learn more here).

Well, the same researchers are back again, releasing more fluorescent imaging research utilizing our GFP-expressing U87 MG cells. Like in the previous study, the researchers inserted our cells into a mouse model and compared the results to TMR-PEG1k. However, this time, the focus our their study was different.

Image: An illustration of Neuromics’ GFP expressing human glioblastoma cells (U87 MG) compared to the contrast agent TMR-PEG1k in mice.

Neuromics is proud to introduce our newest pioneering research tools - human Cas9 expressing primary cells and GPCR expressing cell lines. With these modifications, the cells are valuable and versatile options for drug discovery, studying disease mechanisms, and countless other applications.

Our Cas9 expressing human primary cells have been genetically modified to express the Cas9 protein in a stable manner. Engineered to continuously produce and maintain the expression of the Cas9 protein, they can be used for precise and consistent CRISPR-based genome editing.

Image: Human retinal microvascular endothelial cells (HRMECs) in culture.

We are starting by offering six types of Cas9 expressing primary cells, including endothelial cells and fibroblasts. However, we will add dozens more in the future. Please contact us if there are any other types you need.

G-Protein Coupled Receptors (GPCRs) are at the forefront of modern drug discovery research, playing a vital role in various signal transduction pathways. We are starting by offering one of the most studied and used cell lines, GPCR expressing CHO-K1 cells.

Our primary human endothelial cells have a distinguished resume of enabling research across a wide spectrum of applications. Just a few months ago, we highlighted the use of our human brain microvascular endothelial cells (HBMECs) (cat. HEC02) in an aging study that looked at cell senescence. However, if there is one area where our endothelial cells stand out, it's in diabetes research.

These cells have been utilized on several occasions to study and learn about how endothelial cells react to different glucose environments. Our endothelial cells were part of a study that proposed a drug to treat diabetic retinopathy. You can review all the ways our endothelial cells are being used here.

Image: Endothelial cells from different vascular beds display heterogeneous mitochondrial network morphology.

Then, just this past week, researchers at the University of Glasgow published a paper using our HBMECs to study diabetes.

Neuromics has a large selection of human cells for cancer research. These cells have been used in impactful and meaningful studies by customers - check all the studies out here. At the center of the selection are our cancer associated fibroblasts (CAFs). Collaborating investigators from Takeda, BioTuring, and BMS utilized our Pancreatic CAFs (cat. CAF118) and CAF Growth Media (cat. CAFM03) in a study released earlier this month.

It is widely accepted that CAFs play a vital role in the tumor microenvironment (TME) and are a frequent target for therapeutics. However, despite successful results in 2D culture, many drug candidates struggle when tested in clinical trials.

Image: Gene expression profile of Neuromics Pancreatic CAFs and BxPC3 cells in 2D and 3D monocultures and cocultures. Different culture conditions lead to changing expression profiles.

In the publication, the investigators examined various culture conditions for CAFs, including 2D and 3D monocultures and cocultures. They found that the expression profile and pro-proliferative effects of CAFs vary substantially depending on the culture condition.

The CAFs show their full potential in the TME when cultured in 3D tumor models. The results suggest that drug discovery research should implement 3D models to get a better-predicted outcome when evaluating drug candidates than 2D culture. Read the full study here.

Our human endothelial cells have always been a particularly versatile tool for researchers. They have been used by neuroscientists to study the blood-brain barrier, diabetes researchers to study diabetic retinopathy, cancer researchers to study tumors, and much more. This week, another study was published using one of our many (see for yourself) types of endothelial cells. You can explore all publications using our endothelial cells here.

Image: A comparison of young and senescent HBMECs looking at DNA damage by staining with DAPI (top), numbers of actin stress fibers (middle), and CD31 expression (bottom)

Our human brain microvascular endothelial cells (HBMECs) (cat. HEC02) were used by scientists at the University of Nottingham in a paper published earlier this week. In the study, the investigators grew our HBMECs until they became senescent with implications in vascular aging and other age-related diseases. They tested our cells to see if they could find any reliable biomarkers for vascular aging by looking at the senescent cells. They noted morphological and functional changes in the HBMECs as the passage number increased. Learn more here.