HRMECs were initiated by elutriation from dissociated normal human retinal tissue. Passage 3 cells are shipped frozen. ENDO-Growth medium is recommended for culture. Cells have an average additional population doubling levels >16 when cultured.
Characterization of the cells
HRMECs are negative for HIV-1, HBV, HCV, and mycoplasma.
|1X Phosphate Buffer Solution||PBS300||100 ml|
|AlphaBioCoat Solution||AC001||20 ml|
|Cell Detachment Solution||ADF001||50 ml|
|ENDO Basal Media||MED002||500 ml|
|ENDO-Growth Media||MED001||500 ml|
|ENDO-Growth Media Kit||EGK001||500 ml|
|ENDO-Growth Supplements||EGS001||25 ml|
|Endothelial Cryopreserve Media||FM300||10 ml|
|Smooth Coat Solution||SC300||20 ml|
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- A. P. Da Cunha, Q. Zhang, M. Prentiss, X. Q. Wu, V. Kainz, Y. Y. Xu, J. Vrouvlianis, H. Li, N. Rangaswamy, B. Leehy, T. L. McGee, C. L. Bell, C. E. Bigelow, V. Kansara, Q. Medley, Q. Huang & H. Y. Wu. The Hierarchy of Proinflammatory Cytokines in Ocular Inflammation. Current Eye Research, Volume 43, 2018 - Issue 4 Published Online: 04 Dec 2017
- Odunayo O. Mugisho, Colin R. Green, Jie Zhang, Nicolette Binz, Monica L. Acosta, Elizabeth Rakoczy and Ilva D. Rupentha. (2017). Immunohistochemical Characterization of Connexin43 Expression in a Mouse Model of Diabetic Retinopathy and in Human Donor Retinas. Int. J. Mol. Sci. doi: 10.3390/ijms18122567
- Michael Anthony Ruiz, Biao Feng, and Subrata Chakrabarti. (2015). Polycomb Repressive Complex 2 Regulates MiR-200b in Retinal Endothelial Cells: Potential Relevance in Diabetic Retinopathy. PLoS One.10(4): e0123987. doi: 10.1371/journal.pone.0123987.
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