Product Details
Catalog Number: HEC09
Applications: Cell Assays
Type: Primary Cells
Storage: Keep frozen in liquid nitrogen until plating
Shipping: Frozen (Dry Ice/Liquid Nitrogen)
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
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500,000+ Cells Frozen$789.00Add to Cart

HRMECs were initiated by elutriation from dissociated normal human retinal tissue. Passage 3 cells are shipped frozen. ENDO-Growth medium is recommended for culture. Cells have an average additional population doubling levels >16 when cultured.

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence
HRMECs are negative for HIV-1, HBV, HCV, and mycoplasma.
Images
HREMCs

Effect of HG (high glucose) and pro-inflammatory cytokines on connexin43 expression in HRMECs. Immunohistochemical data showing connexin43 expression in (A) normal medium; (B) HG (25 mM); (C) pro-inflammatory cytokines (IL-1β and TNF-α 10 ng/mL each); and (D) a combination of HG and pro-inflammatory cytokines inducing a change in cell morphology with signs of cell swelling, possibly owing to hemichannel opening (indicated by white arrows). https://doi.org/10.1080/02713683.2017.1410180

HREMCs

Effect of HG (high glucose) and pro-inflammatory cytokines on connexin43 expression in HRMECs. Immunohistochemical data showing connexin43 expression in (A) normal medium; (B) HG (25 mM); (C) pro-inflammatory cytokines (IL-1β and TNF-α 10 ng/mL each); and (D) a combination of HG and pro-inflammatory cytokines inducing a change in cell morphology with signs of cell swelling, possibly owing to hemichannel opening (indicated by white arrows). https://doi.org/10.1080/02713683.2017.1410180

Retinal Microvascular Endothelial Cells in culture

Retinal Microvascular Endothelial Cells in culture

Retinal Microvascular Endothelial Cells in culture

Retinal Microvascular Endothelial Cells in culture