Product Details
Catalog Number: HEC02
Applications: Cell Assays
Type: Primary Cells
Storage: Keep frozen in liquid nitrogen until plating
Shipping: Frozen (Dry Ice/Liquid Nitrogen)
Format A: Frozen
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
500,000+ Cells frozen$789.00Add to Cart

HBMECs were isolated from normal human brain tissue. Passage 2, cells are ship on dry ice in a vial (also available proliferating culture with a confluency of >90 %, HEC02-T25). ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Characterization of the cells

  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence 

HBMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Date of Expiration: Guaranteed for up to 3 months from date of shipping when stored in liquid nitrogen. We guarantee these cells when cultured according to our protocol and with our coating solution and media.

Product Highlights:
Images
Human Brain Microvascular Endothelial Cells in Culture.

Human Brain Microvascular Endothelial Cells in Culture.

Human Brain Microvascular Endothelial Cells in Culture.

Human Brain Microvascular Endothelial Cells in Culture.

HBMECs stained with CD146

HBMECs stained with CD146

HBMECs stained with Claudin-5

HBMECs stained with Claudin-5

A microscope observed the progress in the development of HBMECs seeded in a microfluidic chip. (A) Fluorescent HBMECs 24 h after being seeded (B) HBMECs 48 h after being seeded shows cell extensions and differentiation gradually; (C, D) the vascular lumen 3D structures were formed along with cell proliferation (Green Fluorescence-Green fluorescence protein). Data courtesy of Yang Wu from Wuhan Polytechnic University (https://doi.org/10.1016/j.ecoenv.2019.110077)

A microscope observed the progress in the development of HBMECs seeded in a microfluidic chip. (A) Fluorescent HBMECs 24 h after being seeded (B) HBMECs 48 h after being seeded shows cell extensions and differentiation gradually; (C, D) the vascular lumen 3D structures were formed along with cell proliferation (Green Fluorescence-Green fluorescence protein). Data courtesy of Yang Wu from Wuhan Polytechnic University (https://doi.org/10.1016/j.ecoenv.2019.110077)