Product Details
Catalog Number: HEC02
Applications: Cell Assays
Type: Primary Cells
Storage: Keep frozen in liquid nitrogen until plating
Shipping: Frozen (Dry Ice/Liquid Nitrogen)
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
500,000+ Cells frozen$789.00Add to Cart

HBMECs were isolated from normal human brain tissue. Passage 2, cells are ship on dry ice in a vial (also available proliferating culture with a confluency of >90 %, HEC02-T25). ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Characterization of the cells

  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence 

HBMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Date of Expiration: Guaranteed for up to 6 months from date of shipping when stored in liquid nitrogen. We guarantee these cells when cultured according to our protocol and with our coating solution and media. 

Images
Human Brain Microvascular Endothelial Cells in Culture.

Human Brain Microvascular Endothelial Cells in Culture.

Human Brain Microvascular Endothelial Cells in Culture.

Human Brain Microvascular Endothelial Cells in Culture.