HSIMECs initiated by elutriation from dissociated normal small intestine tonsil tissue. Passage 3 cells are ship in proliferating culture with a confluency of >90 %. ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >16 when cultured.
Characterization of the cells
HSIMECs are negative for HIV-1, HBV, HCV, and mycoplasma.
**cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. As the cells are shipped live, extreme weather (including extreme cold or heat) can be a factor if we are able to ship or not.
|1X Phosphate Buffer Solution||PBS300||100 ml|
|AlphaBioCoat Solution||AC001||20 ml|
|Cell Detachment Solution||ADF001||50 ml|
|ENDO Basal Media||MED002||500 ml|
|ENDO-Growth Media||MED001||500 ml|
|ENDO-Growth Media Kit||EGK001||500 ml|
|ENDO-Growth Supplements||EGS001||25 ml|
|Endothelial Cryopreserve Media||FM300||10 ml|
|Smooth Coat Solution||SC300||20 ml|