Product Details
Catalog Number: HEC15-T25
Applications: Cell Assays
Type: Primary Cells
Storage: 37°C Incubator
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
500,000+ Cells in T25 Flask$789.00Add to Cart

HSIMECs initiated by elutriation from dissociated normal small intestine tonsil tissue. Passage 3 cells are ship in proliferating culture with a confluency of >90 %. ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >16 when cultured.

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence

HSIMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

**cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. As the cells are shipped live, extreme weather (including extreme cold or heat) can be a factor if we are able to ship or not. 

Images
HEC15

Staining of cells with CD31 in red and vWF in green. Cells were counterstained with DAPI (cell nuclei). CD31 is distributed on cell membrane whereas vWF is in cytoplasm as expected.

HEC15

Staining of cells with CD31 in red and vWF in green. Cells were counterstained with DAPI (cell nuclei). CD31 is distributed on cell membrane whereas vWF is in cytoplasm as expected.