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Immunofluorescent analysis of a section of adult rat hippocampus stained with goat pAb to Fox3/NeuN, GT22106, dilution 1:2,000 in red, costained with mouse monoclonal antibdy to MAP2 in green. Nuclear DNA was revealed in blue using the DAPI stain. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.
Immunofluorescent analysis of a section of adult rat hippocampus stained with goat pAb to Fox3/NeuN, GT22106, dilution 1:2,000 in red, costained with mouse monoclonal antibdy to MAP2 in green. Nuclear DNA was revealed in blue using the DAPI stain. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.
Western blot analysis of whole brain lysates using goat pAb to FOX3/NeuN, GT22106, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain. Bands at 46k and 48 kDa correspond to protein isotypes of FOX3/NeuN.
Western blot analysis of whole brain lysates using goat pAb to FOX3/NeuN, GT22106, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain. Bands at 46k and 48 kDa correspond to protein isotypes of FOX3/NeuN.