GFP expressing human umbilical vein endothelial cells were isolated from normal human umbilical vein and transfected with GFP-lentiviral particles at passage 1. Puromycin resistant GFP-HUVECs are passage 3 and are shipped frozen (or in proliferating culture with a confluency of >90 %, GF01-T25). ENDO-Growth Media (MED001) is recommended for culture. Cells have an average additional population doubling levels >18 when cultured.
Characterization of the cells
HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma.
|1X Phosphate Buffer Solution||PBS300||100 ml|
|AlphaBioCoat Solution||AC001||20 ml|
|Cell Detachment Solution||ADF001||50 ml|
|ENDO Basal Media||MED002||500 ml|
|ENDO-Growth Media||MED001||500 ml|
|ENDO-Growth Media Kit||EGK001||500 ml|
|ENDO-Growth Supplements||EGS001||25 ml|
|Endothelial Cryopreserve Media||FM300||10 ml|
|Smooth Coat Solution||SC300||20 ml|
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- Yan Li, Chuanlin Hu, Pengcheng Wang, Yan Liu, Luyang Wang, Qingmeng Pi, Zhiyong Gong, Xu Yang, Michael Mak and Yang Wu (2019). Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip. Journal of Nanobiotechnology 2019 17:20. https://doi.org/10.1186/s12951-019-0458-2
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