Product Details
Catalog Number: 2-1252-001
Applications: Protein Purification, Gravity Flow
Storage: 2-8°C
Shipping: Room Temperature
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
1 ml$149.00Add to Cart
5 x 1 ml$710.00Add to Cart

On 10/31/2024, Strep-Tactin® Sepharose® and Strep-Tactin® Superflow® products were discontinued. The replacement product is Strep-Tactin® 4Flow® High Capacity Products. Learn more below.

Strep-Tactin® 4Flow® high capacity columns are designed for purifying Strep-tag®II and Twin-Strep-tag® proteins via affinity chromatography in gravity flow. This product features a 4% cross-linked agarose beads coupled with Strep-Tactin®, a variant of streptavidin. Strep-Tactin® selectively binds to proteins fused with a Strep-tag®II or Twin-Strep-tag® and enables targeted elution via desthiobiotin. It can be regenerated with 100 mM NaOH for multiple uses, offering a cost-effective solution. This product serves as an alternative to our Strep-Tactin®XT 4Flow® resins for proteins that are challenging to elute.

Replacing:

Name

Catalog Number

Strep-Tactin® Sepharose® Column

2-1202-001

Strep-Tactin® Superflow® High Capacity Column

2-1209-001

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Sepharose® and Strep-Tactin® Superflow®:

 Matrix

 Strep-Tactin® Sepharose®

Strep-Tactin® Superflow®

Strep-Tactin® 4Flow®

Bead structure

4% agarose, crosslinked

6% agarose, crosslinked

4% agarose, highly crosslinked

Bead size

45-165 um

60-160 um

50-150 um, spherical

Exclusion limit

~3 x 10^7 Da

6 x 10^6 Da

3 x 10^7 Da

Recommended flow rate

gravity flow

0.5-1 ml/min

0.5-1 ml/min

pH range for protein binding

7-8

7-8

7-8

Max pressue

gravity flow

9.6 bar

3.5 bar

 Eluent

desthiobiotin

desthiobiotin

desthiobiotin

Regeneration buffer

Buffer R

Buffer R

100 mM NaOH

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Neuromics
5325 West 74th St., Suite 8
Edina, MN 55439

Toll free: 866-350-1500
Int’l phone: 952-374-6161
Fax: 612-677-3976