The pASG-IBA43 vector allows the expression of 6xHistidine-/ Strep-tag®-fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the 6xHistidine-tag for N-terminal and the Strep-tag® for C-terminal fusion to the recombinant protein as well as the Ampicillin Resistance cassette. It can be used with any E. coli strain because the tet-promoter works independently from the genetic background of E.coli.
The expressed recombinant protein will be localized in the cytoplasm.
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- Ellen Becher, et al. (2018). Active Site Alanine Preceding Catalytic Cysteine Determines Unique Substrate Specificity in Bacterial CoA-Acylating Prenal Dehydrogenase. FEBS Letters, 592(7), 1150-1160. doi: 10.1002/1873-3468.13019
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