The pASG-IBA144 vector allows the expression of Twin-Strep®-/6xHistidine-tag-fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the ompA signal for periplasmic secretion of the recombinant protein. Additionally it contains the Twin-Strep-tag® for N-terminal and the 6xHistidine-tag for C-terminal fusion to the recombinant protein as well as the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli.
StarGate® Acceptor Vector for bacterial expression.
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