The pASG-IBAwt2 vector allows the expression of fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli.
The expressed recombinant protein will be secreted into the periplasm.
This vector does not contain an affinity tag.
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