Product Details
Catalog Number: 5-5102-001
Applications: Gene Transfer
Type: Expression Vector
Species Reactivity: Mammalian
Downloads: Datasheet (pdf)
Downloads: MSDS (pdf)
Product Sizes
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5 µg$268.00Add to Cart

The pCSG-IBA102 vector allows high-level expression of Twin-Strep-tag®-fusion-proteins in mammalian hosts under G418 (Geneticin) selection. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells and the BM40 signal for secretion of the recombinant protein and the Twin-Strep-tag® for C-terminal fusion to the recombinant protein. The episomal replication is mediated through the Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). For selection and propagation in E.coli the vector carries an Ampicillin resistance and ColE1 origin of replication (pUC). The expressed recombinant protein will be secreted into the medium.

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