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Images
Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding full length CasΦ protein and stained with rabbit pAb against CasΦ, RA22145, in red. The blue is Hoechst staining of nuclear DNA. The antibody reveals cytoplasmic expression of the CasΦ protein only in transfected cells.
Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding full length CasΦ protein and stained with rabbit pAb against CasΦ, RA22145, in red. The blue is Hoechst staining of nuclear DNA. The antibody reveals cytoplasmic expression of the CasΦ protein only in transfected cells.
Western blot analysis of HEK293 cell lysates using rabbit pAb to CasΦ protein, RA22145, dilution 1:1,000 in green: [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to the expected GFP-CasΦ fusion protein.
Western blot analysis of HEK293 cell lysates using rabbit pAb to CasΦ protein, RA22145, dilution 1:1,000 in green: [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to the expected GFP-CasΦ fusion protein.