There has been much recent interest in gene editing and other genetic manipulations by CRISPR-Cas family enzymes. One of these enzymes is referred to as CasΦ and also known as Cas-12j. The use of such smaller enzymes leaves more room for other nucleic acid sequences in AAV and other viral vectors which typically have a limited DNA capacity, thus allowing more versatility for new CRISPR based manipulations.
RA22145 was made against a recombinant construct of the full sequence of CasΦ-2. The antibody works well on western blots of crude homogenates of HEK293 cells transfected with the Cas%Phi;-2 DNA, cleanly producing the appropriate sized band. In addition such transfected cells show clean and strong cytoplasmic staining by immunofluorescence staining with this antibody.