Product Details
Catalog Number: RA22145
Applications: ICC, WB, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:2,000-5,000; Western Blot: 1:1,000-2,000
Immunogen: Full length Cas?-2 expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Not Applicable
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$299.00Add to Cart

There has been much recent interest in gene editing and other genetic manipulations by CRISPR-Cas family enzymes. One of these enzymes is referred to as CasΦ and also known as Cas-12j. The use of such smaller enzymes leaves more room for other nucleic acid sequences in AAV and other viral vectors which typically have a limited DNA capacity, thus allowing more versatility for new CRISPR based manipulations.

RA22145 was made against a recombinant construct of the full sequence of CasΦ-2. The antibody works well on western blots of crude homogenates of HEK293 cells transfected with the Cas%Phi;-2 DNA, cleanly producing the appropriate sized band. In addition such transfected cells show clean and strong cytoplasmic staining by immunofluorescence staining with this antibody.

Images
Immunofluorescence

Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding full length CasΦ protein and stained with rabbit pAb against CasΦ, RA22145, in red. The blue is Hoechst staining of nuclear DNA. The antibody reveals cytoplasmic expression of the CasΦ protein only in transfected cells.

Immunofluorescence

Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding full length CasΦ protein and stained with rabbit pAb against CasΦ, RA22145, in red. The blue is Hoechst staining of nuclear DNA. The antibody reveals cytoplasmic expression of the CasΦ protein only in transfected cells.

Western Blot

Western blot analysis of HEK293 cell lysates using rabbit pAb to CasΦ protein, RA22145, dilution 1:1,000 in green: [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to the expected GFP-CasΦ fusion protein.

Western Blot

Western blot analysis of HEK293 cell lysates using rabbit pAb to CasΦ protein, RA22145, dilution 1:1,000 in green: [1] protein standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to the expected GFP-CasΦ fusion protein.