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Images
Transfected HEK293 cells overexpressing a RA22127 fusion protein were stained with RA22127. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining with RA22127 is shown in red in middle panel. In merged image (right panel), most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.
Transfected HEK293 cells overexpressing a RA22127 fusion protein were stained with RA22127. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining with RA22127 is shown in red in middle panel. In merged image (right panel), most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.






