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Name Catalog # Size CasΦ/Cas12j MO22209 100 ul CasΦ/Cas12j RA22145 100 ul Cas9 from S. pyogenes RA22126 100 ul Cas9 from Staphylococcus aureus CH22120 100 ul Cas9 from Staphylococcus aureus MO22175 100 ul Cas9 from Staphylococcus aureus RA22127 100 ul
Images
Transfected Hek293 cells overexpressing the N-terminal 1-608 amino acids of S. pyogenes Cas9 . The cells were stained with MO22174 in red, and these cells also appear yellow since we counterstained with our rabbit Cas9 antibody, RA22126 to the same construct in green, giving a yellow color. Most Hek293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain
Transfected Hek293 cells overexpressing the N-terminal 1-608 amino acids of S. pyogenes Cas9 . The cells were stained with MO22174 in red, and these cells also appear yellow since we counterstained with our rabbit Cas9 antibody, RA22126 to the same construct in green, giving a yellow color. Most Hek293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain
Lane 1: Protein size marker with size in kiloDaltons.. Lane 2: Blot of crude protein extract from Hek293 culture transfected with MO22174 immunogen, the N-terminal 1-608 amino acids. Lane 3: Non-transfected control Hek293 cell extract. Lane 4: Blot of 40 ng Streptococcus pyogenes Cas9 was probed with MO22174 at 1:1,000 dilution and, as expected, the antibody also recognizes full length Cas9 protein at 160 kDa.
Lane 1: Protein size marker with size in kiloDaltons.. Lane 2: Blot of crude protein extract from Hek293 culture transfected with MO22174 immunogen, the N-terminal 1-608 amino acids. Lane 3: Non-transfected control Hek293 cell extract. Lane 4: Blot of 40 ng Streptococcus pyogenes Cas9 was probed with MO22174 at 1:1,000 dilution and, as expected, the antibody also recognizes full length Cas9 protein at 160 kDa.