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Images
Transfected HEK293 cells which overexpressing GFP-Cas9-SA fusion protein were stained with CH22120. Cells which are transfected with GFP-Cas9 are bright green (left). Staining with CH22120 is shown in red (middle). Merged image (right), most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.
Transfected HEK293 cells which overexpressing GFP-Cas9-SA fusion protein were stained with CH22120. Cells which are transfected with GFP-Cas9 are bright green (left). Staining with CH22120 is shown in red (middle). Merged image (right), most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.