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Images
Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector (5) including DNA encoding the CasΦ protein, stained with mouse mAb against CasΦ, MO22209, dilution 1:2,000, in red, and costained with rabbit pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. MO22209 reveals expression of the CasΦ protein only in transfected cells. The vimentin antibody stains the cytoskeletal intermediate filament network in all cells.
Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector (5) including DNA encoding the CasΦ protein, stained with mouse mAb against CasΦ, MO22209, dilution 1:2,000, in red, and costained with rabbit pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. MO22209 reveals expression of the CasΦ protein only in transfected cells. The vimentin antibody stains the cytoskeletal intermediate filament network in all cells.
Western blot analysis of HEK293 cell lysates using mouse mAb to CasΦ protein, MO22209, dilution 1:1,000 in green: [1] protein molecular weight standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to GFP-CasΦ fusion protein.
Western blot analysis of HEK293 cell lysates using mouse mAb to CasΦ protein, MO22209, dilution 1:1,000 in green: [1] protein molecular weight standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to GFP-CasΦ fusion protein.