Product Details
Catalog Number: MO22209
Applications: ICC, WB, IF
Type: Mouse IgG
Dilutions: Immunofluorescence: 1:1,000; Western Blot: 1:1,000-2,000
Immunogen: Recombinant Cas?/Cas-12j expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Not Applicable
Downloads: Datasheet (pdf)
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100 ul$275.00Add to Cart

There has been much recent interest in gene editing and other genetic manipulations by CRISPR-Cas family enzymes. One of these enzymes is referred to as CasΦ and also known as Cas-12j. The use of such smaller enzymes leaves more room for other nucleic acid sequences in AAV and other viral vectors which typically have a limited DNA capacity, thus allowing more versatility for new CRISPR based manipulations.

MO22209 antibody was made against a recombinant construct of the full sequence of CasΦ-2. The antibody works well on western blots of crude homogenates of HEK293 cells transfected with the Cas-Φ-2 DNA, cleanly producing the appropriate sized band. In addition, such transfected cells show clean and strong cytoplasmic staining by immunofluorescence staining with this antibody.

Images
Immunofluorescence

Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector (5) including DNA encoding the CasΦ protein, stained with mouse mAb against CasΦ, MO22209, dilution 1:2,000, in red, and costained with rabbit pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. MO22209 reveals expression of the CasΦ protein only in transfected cells. The vimentin antibody stains the cytoskeletal intermediate filament network in all cells.

Immunofluorescence

Immunofluorescent analysis of HEK293 cells transfected with pCI-Neo-Mod vector (5) including DNA encoding the CasΦ protein, stained with mouse mAb against CasΦ, MO22209, dilution 1:2,000, in red, and costained with rabbit pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. MO22209 reveals expression of the CasΦ protein only in transfected cells. The vimentin antibody stains the cytoskeletal intermediate filament network in all cells.

Western Blot

Western blot analysis of HEK293 cell lysates using mouse mAb to CasΦ protein, MO22209, dilution 1:1,000 in green: [1] protein molecular weight standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to GFP-CasΦ fusion protein.

Western Blot

Western blot analysis of HEK293 cell lysates using mouse mAb to CasΦ protein, MO22209, dilution 1:1,000 in green: [1] protein molecular weight standard, [2] non-transfected cells, [3] cells transfected with pCI-Neo-Mod vector containing full length CasΦ cDNA, and [4] cells transfected with pCI-Neo-GFP vector containing full length CasΦ cDNA. The band at about 90kDa demonstrates expression of CasΦ protein, and the band at about 120kDa corresponds to GFP-CasΦ fusion protein.