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Name Catalog # Size CasΦ/Cas12j MO22209 100 ul CasΦ/Cas12j RA22145 100 ul Cas9 from Staphylococcus aureus CH22120 100 ul Cas9 from Staphylococcus aureus MO22175 100 ul Cas9 from Staphylococcus aureus RA22127 100 ul Cas9 from Streptococcus pyogenes MO22174 100 ul
Images
Hek293 cell culture transfected with a construct including the C terminal 814-1372 amino acids of Staphylococcus pyogenes Cas9. The cells were stained with RA22126 in red and also with a chicken antibody to S. pyogenes Cas9 in green. A cell expressing Cas9 therefore appears yellow. Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with the blue DNA stain.
Hek293 cell culture transfected with a construct including the C terminal 814-1372 amino acids of Staphylococcus pyogenes Cas9. The cells were stained with RA22126 in red and also with a chicken antibody to S. pyogenes Cas9 in green. A cell expressing Cas9 therefore appears yellow. Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with the blue DNA stain.
Western blot analysis of RA22126 Blot of 100 ng (lane1) and 10 ng (lane2) of full length Cas9 protein from Streptococcus pyogenes was probed with RA22126 at 1:2,000 dilution. This antibody recognizes full length Cas9 protein at 160 kDa and a proteolytic fragment at 130 kDa.
Western blot analysis of RA22126 Blot of 100 ng (lane1) and 10 ng (lane2) of full length Cas9 protein from Streptococcus pyogenes was probed with RA22126 at 1:2,000 dilution. This antibody recognizes full length Cas9 protein at 160 kDa and a proteolytic fragment at 130 kDa.