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Name Catalog # Size CasΦ/Cas12j MO22209 100 ul CasΦ/Cas12j RA22145 100 ul Cas9 from S. pyogenes RA22126 100 ul Cas9 from Staphylococcus aureus CH22120 100 ul Cas9 from Staphylococcus aureus RA22127 100 ul Cas9 from Streptococcus pyogenes MO22174 100 ul
Images
Transfected HEK293 cells with GFP-Cas9-SA fusion protein were stained with MO22175 antibody. GFP is expressed in the transfected cells (green). GFP-Cas9 fusion protein is stained with MO22157 antibody at 1:1,000 dilution (red). Only transfected cells are reactive with MO22157 antibody, which appear in a yellow-orange color (merge). Nuclei are visualized in blue with Hoechst staining.
Transfected HEK293 cells with GFP-Cas9-SA fusion protein were stained with MO22175 antibody. GFP is expressed in the transfected cells (green). GFP-Cas9 fusion protein is stained with MO22157 antibody at 1:1,000 dilution (red). Only transfected cells are reactive with MO22157 antibody, which appear in a yellow-orange color (merge). Nuclei are visualized in blue with Hoechst staining.
Image: Western blot analysis of MO22175 1: HEK293 cells which overexpressed fusion protein containing GFP and c-terminus of Cas9 from S. aureus. 2: Non-transfected HEK293 cells. There is a strong clean band at about 53 kDa corresponding to GFP-Cas9 fusion protein, which is absent in non-transfected cells. MCA-6F7 antibody is diluted at 1: 1,000 dilution
Image: Western blot analysis of MO22175 1: HEK293 cells which overexpressed fusion protein containing GFP and c-terminus of Cas9 from S. aureus. 2: Non-transfected HEK293 cells. There is a strong clean band at about 53 kDa corresponding to GFP-Cas9 fusion protein, which is absent in non-transfected cells. MCA-6F7 antibody is diluted at 1: 1,000 dilution