Product Details
Catalog Number: MO22165
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Immunogen: Full length recombinant human protein; HGNC name for this protein is CALB2
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine
Entrez: 794
UniProt: P22676
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$325.00Add to Cart

Calretinin is a member of the large superfamily of cytoplasmic Ca2+ binding proteins, thus belongs to the subclass of these containing the “EF hand” Ca2+ binding motif originally characterized in parvalbumin. Calretinin is expressed in mammalian central nerve system, testis, fallopian tube and pancreas. In the brain it is localized in certain classes of neurons, and antibodies to it are useful for identifying specific neuronal cell types. It is particularly concentrated in some cerebellar granular cells and their parallel fibers, but is also found in many GABAergic interneurons in the cortex. These GABAergic interneurons, in most cases, express only one of three Ca2+ binding proteins, namely calretinin, calbindin or parvalbumin . As a result, these important inhibitory interneurons can be identified and subclassified based on their content of these three proteins. Each type of neuron as defined in this fashion has particular electrophysiological and functional properties. For example, calbindin positive interneurons are not fast-spiking as are parvalbumin expressinginterneurons. Human calretinin is also known as 29 kDa calbindin and calbindin-2, for its sequence is related to calbindin.

Images

Left: Adult mouse brain hippocampal section (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with Calretinin MO22165 (1:1000, red) and our rabbit polyclonal antibody to Fox3/NeuN (RA22113, green). Right: Adult rat cortical section (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with MO22165 (red) and our chicken polyclonal antibody to calbinidin (CH22118, green).

Left: Adult mouse brain hippocampal section (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with Calretinin MO22165 (1:1000, red) and our rabbit polyclonal antibody to Fox3/NeuN (RA22113, green). Right: Adult rat cortical section (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with MO22165 (red) and our chicken polyclonal antibody to calbinidin (CH22118, green).