Product Details
Catalog Number: CH22137
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:500-1,000; Immunohistochemistry: 1:500-1,000; Western Blot: 1:5,000-10,000
Immunogen: Full length recombinant human MARCKS expressed in and purified from E. coli.
Storage: Store at 4°C.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Primate
Entrez: 4082
UniProt: P29966
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered by as a major substrate for protein kinase C in the brain and other tissues. MARCKS interacts with actin and calcium in a manner regulated by protein kinase C. It is a major protein of the brain concentrated in the synapses of neurons and is membrane localized due to the N-terminal lipid myristoyl group. Antibody to MARCKS can therefore be used as a marker of synaptic regions. Genetic knock out of MARCKS in transgenic mice is perinatal lethal and associated with aberrant brain development suggesting a fundamental importance in the CNS.

This antibody was made against recombinant full length human MARCKS expressed in and purified from E. coli. The antibody works well on human cells and tissues but is not recommended for work on rodent material.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to MARCKS, CH22137, dilution 1:5,000 in red, and costained with mouse mAb to β-tubulin in green. The blue is Hoechst staining of nuclear DNA. The MARCKS antibody binds to MARCKS protein expressed in the plasma membrane and cytoplasm, while the β-tubulin antibody stains cytoplasmic microtubules.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with chicken pAb to MARCKS, CH22137, dilution 1:5,000 in red, and costained with mouse mAb to β-tubulin in green. The blue is Hoechst staining of nuclear DNA. The MARCKS antibody binds to MARCKS protein expressed in the plasma membrane and cytoplasm, while the β-tubulin antibody stains cytoplasmic microtubules.

Western Blot

Western blot analysis of different cell lines lysates using chicken pAB to MARCKS, CH22137, dilution 1:1,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, and [5] COS1 cells. Strong band at 80kDa corresponds to MARCKS protein. The same blot was simultaneously probed with mouse mAb to GAPDH in red, which reveals a single band at 37kDa in all preparations.

Western Blot

Western blot analysis of different cell lines lysates using chicken pAB to MARCKS, CH22137, dilution 1:1,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, and [5] COS1 cells. Strong band at 80kDa corresponds to MARCKS protein. The same blot was simultaneously probed with mouse mAb to GAPDH in red, which reveals a single band at 37kDa in all preparations.