Images

Left: Mixed neuron/glial cultures stained with MO22166 at 1:2,000 in red, and our chicken polyclonal antibody to Vimentin (CH22108) in green. Middle: Adult rat cortical section was co-stained with MO22166 (1:1000; red) and our chicken polyclonal antibody to calbindin (CH22118, green). Right: Adult mouse brain hippocampal section (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with MO22166 (1:1000, red) and our calbindin CH22118, (green)

Left: Mixed neuron/glial cultures stained with MO22166 at 1:2,000 in red, and our chicken polyclonal antibody to Vimentin (CH22108) in green. Middle: Adult rat cortical section was co-stained with MO22166 (1:1000; red) and our chicken polyclonal antibody to calbindin (CH22118, green). Right: Adult mouse brain hippocampal section (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with MO22166 (1:1000, red) and our calbindin CH22118, (green)

Western blot analysis of MO22166. Blot of rat brain lysates (lane1), recombinant proteins: pavalbumin (lane 2), calretinin (lane 3), calbindin ( lane 4) was probed with MO22166 at 1:5,000. In rat brain lysates, this antibody recognizes a clean band at 29 kDa which represents calretinin . Also it reacts with only calretinin, not other calcium-binding proteins.

Western blot analysis of MO22166. Blot of rat brain lysates (lane1), recombinant proteins: pavalbumin (lane 2), calretinin (lane 3), calbindin ( lane 4) was probed with MO22166 at 1:5,000. In rat brain lysates, this antibody recognizes a clean band at 29 kDa which represents calretinin . Also it reacts with only calretinin, not other calcium-binding proteins.

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Neuromics
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