Product Details
Catalog Number: MO22166
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine
Entrez: 794
UniProt: P22676
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

Calretinin is a member of the large superfamily of cytoplasmic Ca2+ binding proteins, thus belongs to the subclass of these containing the “EF hand” Ca2+ binding motif originally characterized in parvalbumin. Calretinin is expressed in mammalian central nerve system, testis, fallopian tube and pancreas. In the brain it is localized in certain classes of neurons, and antibodies to it are useful for identifying specific neuronal cell types. It is particularly concentrated in some cerebellar granular cells and their parallel fibers, but is also found in many GABAergic interneurons in the cortex. These GABAergic interneurons, in most cases, express only one of three Ca2+ binding proteins, namely calretinin, calbindin or parvalbumin . As a result, these important inhibitory interneurons can be identified and subclassified based on their content of these three proteins. Each type of neuron as defined in this fashion has particular electrophysiological and functional properties. For example, calbindin positive interneurons are not fast-spiking as are parvalbumin expressinginterneurons. Human calretinin is also known as 29 kDa calbindin and calbindin-2, for its sequence is related to calbindin.

Images

Left: Mixed neuron/glial cultures stained with MO22166 at 1:2,000 in red, and our chicken polyclonal antibody to Vimentin (CH22108) in green. Middle: Adult rat cortical section was co-stained with MO22166 (1:1000; red) and our chicken polyclonal antibody to calbindin (CH22118, green). Right: Adult mouse brain hippocampal section (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with MO22166 (1:1000, red) and our calbindin CH22118, (green)

Left: Mixed neuron/glial cultures stained with MO22166 at 1:2,000 in red, and our chicken polyclonal antibody to Vimentin (CH22108) in green. Middle: Adult rat cortical section was co-stained with MO22166 (1:1000; red) and our chicken polyclonal antibody to calbindin (CH22118, green). Right: Adult mouse brain hippocampal section (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) was co-stained with MO22166 (1:1000, red) and our calbindin CH22118, (green)

Western blot analysis of MO22166. Blot of rat brain lysates (lane1), recombinant proteins: pavalbumin (lane 2), calretinin (lane 3), calbindin ( lane 4) was probed with MO22166 at 1:5,000. In rat brain lysates, this antibody recognizes a clean band at 29 kDa which represents calretinin . Also it reacts with only calretinin, not other calcium-binding proteins.

Western blot analysis of MO22166. Blot of rat brain lysates (lane1), recombinant proteins: pavalbumin (lane 2), calretinin (lane 3), calbindin ( lane 4) was probed with MO22166 at 1:5,000. In rat brain lysates, this antibody recognizes a clean band at 29 kDa which represents calretinin . Also it reacts with only calretinin, not other calcium-binding proteins.