Product Details
Catalog Number: RA22111
Applications: ICC, WB, IF
Type: Rabbit IgG
Dilutions: ICC 1:1000; IHC 1:1000; WB 1:10,000
Immunogen: Purified recombinant MARCKS protein.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Mouse, Rat
Entrez: 4082
UniProt: P29966
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death. It plays a vital role in the normal developmental processes of neurulation, hemisphere fusion, forebrain commissure formation, and formation of cortical and retinal laminations.

Images

View of mixed neuron/glial cultures stained with our MARCKS antibody (red) and MAP2 made in chicken. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.

View of mixed neuron/glial cultures stained with our MARCKS antibody (red) and MAP2 made in chicken. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.

Western blot of whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with RPCA-MARCKS, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~80kDa corresponds to MARCKS.

Western blot of whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with RPCA-MARCKS, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~80kDa corresponds to MARCKS.