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Immunofluorescent analysis of mouse cerebellum section stained with chicken pAb to FOX2, CH22130, dilution 1:2,000 in red, and costained with mouse mAb to NF-L in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained with above antibodies.
Immunofluorescent analysis of mouse cerebellum section stained with chicken pAb to FOX2, CH22130, dilution 1:2,000 in red, and costained with mouse mAb to NF-L in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained with above antibodies.
Western blot analysis of nuclear enriched fractions from different tissue and cells using chicken pAb to FOX2, CH22130, in green: [1] protein standard , [2] rat brain, [3] mouse brain, [4] HEK293, and [5] NIH-3T3 cells. The bands between 50kDa and 75kDa correspond to the FOX2 protein.
Western blot analysis of nuclear enriched fractions from different tissue and cells using chicken pAb to FOX2, CH22130, in green: [1] protein standard , [2] rat brain, [3] mouse brain, [4] HEK293, and [5] NIH-3T3 cells. The bands between 50kDa and 75kDa correspond to the FOX2 protein.