Ready to Grow Fibroblasts
HNDFs are isolated from normal neonatal forehead skin tissue samples and transfected with GFP-Lentiviral particles at passage one. Puromycin resistant GPF-HNDFs are selected and shipped in proliferating culture with >90 confluence (the cells are provided @ passage 3). DMEM contains 5% Fetal calf serum (Full medium) is recommended for cell culture and these cells have an average minimum population doubling levels > 8. GFP-HNDFs are tested negative for HIV-1, HBV, HCV, and mycoplasma.