i-BRITE Plus is a mounting medium which significantly extends a light-emitting capacity of fluorescent dyes which tend to fade during storage and microscopy examination. i-BRITE Plus can be used for various applications including, but not limited to, fluorescence imunohistochemistry and cytochemistry (using, for example, FITC, Cy® and Alexa® dyes), fluorescence neuronal tracing, and gene expression studies (using, for example, GFP and YFP tags). i-BRITE Plusmedium is compatible with cryostat and paraffin-embedded tissue sections, as well as with cultured neuronal and non-neuronal cells. Unlike other types of mounting media, I-BRITE Plus protects cells and tissues from shrinking thus maintaining their native cytological and histological architecture, which is of critical importance for accurate image analysis studies
i-BRITE Plus blocks fading of a large variety of fluorescent dyes within a broad range of excitation and emission spectra, which makes this mounting medium a reagent of choice for maintaining brightness of multi-color fluorescence samples during storage and analysis by either conventional fluorescence or laser confocal microscopy.