Gravity flow Strep-Tactin® Sepharose® column

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Laura Prioretti, et al. (2023). Carbon Fixation in the Chemolithoautotrophic Bacterium Aquifex aeolicus Involves Two Low-Potential Ferredoxins as Partners of the PFOR and OGOR Enzymes. Life, 13(3), 627. doi: 10.3390/life13030627
Yun Nan Hou, et al. (2022). A Conformation-Specific Nanobody Targeting the Nicotinamide Mononucleotide-Activated State of SARM1. Nature Communications, 13, 7898. doi: 10.1038/s41467-022-35581-y
Lukas Muschallik, et al. (2020). Synthesis of α-hydroxy Ketones and Vicinal (R,R)-Diols by Bacillus clausii DSM 8716T Butanediol Dehydrogenase. RSC Advances, 10, 12206-12216. doi: 10.1039/D0RA02066D
Christina S. Heil, et al. (2018). Site-Specific Labeling of Multidomain Proteins by Amber Codon Suppression. Scientific Reports, 8, 14864. doi: 10.1038/s41598-018-33115-5
Christophe J. Lalaurie, et al. (2018). The de novo Design of a Biocompatible and Functional Integral Membrane Protein Using Minimal Sequence Complexity. Scientific Reports, 8, 14564. doi: 10.1038/s41598-018-31964-8
Nicole Schäfer, et al. (2016). Complement Regulator FHR-3 Is Elevated either Locally or Systemically in a Selection of Autoimmune Diseases. Frontiers in Immunology, 7, doi: 10.3389/fimmu.2016.00542
Cyril Barinka, et al. (2016). Selection and Characterization of Anticalins Targeting Human Prostate-Specific Membrane Antigen (PSMA). Protein Engineering, Design and Selection, 29(3), 105–115. doi: 10.1093/protein/gzv065
Sebastian Dintner, et al. (2014). A Sensory Complex Consisting of an ATP-binding Cassette Transporter and a Two-component Regulatory System Controls Bacitracin Resistance in Bacillus subtilis. Journal of Biological Chemistry, 289(40), 27899-27910. doi: 10.1074/jbc.M114.596221

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A 36 kDa enzyme and a mutant, both with C-terminal Strep-tag II, were expressed in the cytoplasm of E. coli. 1: Sample of crude lysate after cytosolic expression with pASK-IBA3 2: Sample of flow through during chromatography 3: Sample after the addition of 2.5 mM desthiobiotin M: Molecular size standard (kDa)

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