Product Details
Catalog Number: 2-1501-001
Applications: E, Fluoro-Imaging
Type: Chromogenic Detection
Species Reactivity: Step-Tactin Proteins
Downloads: Datasheet (pdf)
Product Sizes
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1X96 Well Plate$99.00Add to Cart

Ready-to-use Strep-Tactin coated 8-well strips provide the power of our Strep-tag system in a solid-phase, multi-well format for convenient assays and high throughput screenings of biomolecules tagged with Strep-tag II. The strips are supplied framed in sets of 12, resulting in a 96-well configuration compatible with standard multichannel pipettes and automated plate washers and plate readers.

Capablilties:
  • Oriented immobilization of recombinant proteins with N-terminal, C-terminal or internal Strep-tag II
  • Effective screening procedures
  • Minimal non-specific binding High and constant activity
Strep-tag® fusion protein binding capacity100 ng Strep-tag II fusion protein/well
Intra- and inter-assay variance (cv)< 1.6% and < 3.0%, respectively
Max. volume per well300 µl
Optimal pH8.0
Stability> 1 year at 4 °C
Storage4 °C
ShipmentRT
Images

Strep-Tactin coated microplate was incubated with recombinant H. pylori urease/Strep-tag II fusion protein, followed by 3 washing cycles. 2. Second incubation with human sera followed by 3 washing cycles. 3. Third incubation with rabbit anti-human lgG conjugated to peroxidase, followed by 3 washing cycles. 4. Colorimetric determination of bound peroxidase activity.

Strep-Tactin coated microplate was incubated with recombinant H. pylori urease/Strep-tag II fusion protein, followed by 3 washing cycles. 2. Second incubation with human sera followed by 3 washing cycles. 3. Third incubation with rabbit anti-human lgG conjugated to peroxidase, followed by 3 washing cycles. 4. Colorimetric determination of bound peroxidase activity.