Strep-Tactin® coated microplate

Product Details

Catalog Number: 2-1501-001

Applications: E, Fluoro-Imaging

Type: Chromogenic Detection

Species Reactivity: Step-Tactin Proteins

Product Sizes

Size	List Price	Price	Cart
1X96 Well Plate		$68.00	Add to Cart

Ready-to-use Strep-Tactin coated 8-well strips provide the power of our Strep-tag system in a solid-phase, multi-well format for convenient assays and high throughput screenings of biomolecules tagged with Strep-tag II. The strips are supplied framed in sets of 12, resulting in a 96-well configuration compatible with standard multichannel pipettes and automated plate washers and plate readers.

Capablilties:

Oriented immobilization of recombinant proteins with N-terminal, C-terminal or internal Strep-tag II
Effective screening procedures
Minimal non-specific binding High and constant activity

Strep-tag^® fusion protein binding capacity	100 ng Strep-tag II fusion protein/well
Intra- and inter-assay variance (cv)	< 1.6% and < 3.0%, respectively
Max. volume per well	300 µl
Optimal pH	8.0
Stability	> 1 year at 4 °C
Storage	4 °C
Shipment	RT

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Product Publications

Neeti Ananthaswamy, et al. (2019). A Sequestered Fusion Peptide in the Structure of an HIV-1 Transmitted Founder Envelope Trimer. Nature Communications, 10:873. doi: 10.1038/s41467-019-08825-7
Francisco Romero Pastrana, et al. (2017). A Lactococcus lactis Expression Vector Set with Multiple Affinity Tags to Facilitate Isolation and Direct Labeling of Heterologous Secreted Proteins. Applied Microbiology and Biotechnology, 101, 8139–8149. doi: 10.1007/s00253-017-8524-x
Gleyder Roman-Sosa, et al. (2016). Analysis of the Humoral Immune Response Against the Envelope Glycoprotein Gc of Schmallenberg Virus Reveals a Domain Located At the Amino Terminus Targeted by mAbs with Neutralizing Activity. Journal of General Virology, 97(3). doi: 10.1099/jgv.0.000377
Wadad Al Salmi, et al. (2015). A New Approach to Produce HIV-1 Envelope Trimers. Journal of Biological Chemistry, 290(32), 19780-19795. doi: 10.1074/jbc.M115.656611
Wolfgang J. Ernst, et al. (2001). Expanding Baculovirus Surface Display. European Journal of Biochemistry, 267(13), 4033-4039. doi: 10.1046/j.1432-1327.2000.01439.x

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StrepMAB-Immo DY-549	2-1567-050	5 x 50 µg
StrepMAB-Immo DY-549	2-1567-050	50 µg
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Images

Strep-Tactin coated microplate was incubated with recombinant H. pylori urease/Strep-tag II fusion protein, followed by 3 washing cycles. 2. Second incubation with human sera followed by 3 washing cycles. 3. Third incubation with rabbit anti-human lgG conjugated to peroxidase, followed by 3 washing cycles. 4. Colorimetric determination of bound peroxidase activity.