Strep-Tactin® AP Conjugate (Strep-Tactin® labeled with alkaline phosphatase)

Product Details

Catalog Number: 2-1503-001

Applications: WB

Type: Chromogenic Detection

Format A: liquid

Species Reactivity: Step-Tactin Proteins

Downloads: Western Blot Protocol (pdf)

Downloads: SDS (pdf)

Downloads: Datasheet (pdf)

Product Sizes

Size	List Price	Price	Cart
0.5 ml		$169.00	Add to Cart

Specifications of Strep-Tactin AP conjugate

Alkaline Phosphatase	Calf intestine
Detection	N- or C-terminal or internal Strep-tag II
Sensitivity in dot blots	> 1 ng/dot
Sensitivity in Western blots	> 2 ng/band
Cross reactivity	Reacts with Biotin Carboxyl Carrier Protein (BCCP, 22.5 kDa) in E. coli extracts. Generally, biotinylated proteins can be sufficiently masked by a 10 minute preincubation with 2 µg/ml Biotin Blocking Buffer
Form	Suspended in 0.01 M phospate buffered saline pH 7.4 containing BSA and 0.01 % Thimerosal as preservative. Can be diluted 1:4,000 for detection of Strep-tag II fusion proteins after Western blotting. (Please note that for ELISA we recommend to use the Strep-Tactin HRP conjugate)
Stability	1 year
Storage	4 °C
Shipment	RT

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Product Publications

Watee Seesuay, et al. (2018). Human Transbodies that Interfere with the Functions of Ebola Virus VP35 Protein in Genome Replication and Transcription and Innate Immune Antagonism. Emerging Microbes & Infections, 7(1), 1-15. doi: 10.1038/s41426-018-0031-3
Cyril Barinka, et al. (2016). Selection and Characterization of Anticalins Targeting Human Prostate-Specific Membrane Antigen (PSMA). Protein Engineering, Design and Selection, 29(3), 105–115. doi: 10.1093/protein/gzv065

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Images

Strep-Tactin® AP conjugate