Product Details
Catalog Number: 3D45001
Applications: In vivo Like Assays
Type: 3D Cell/Tissue Models
Storage: Keep frozen in liquid nitrogen until plating
Shipping: Frozen (Dry Ice/Liquid Nitrogen)
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
12 well$1,399.00Add to Cart
24 well$1,539.00Add to Cart
6 well$1,269.00Add to Cart

Angiogenesis is a multistep process whereby new blood vessels develop from preexisting vasculature. Angiogenesis plays a key role in numerous physiological and pathological processes and understanding the mechanism of angiogenesis will therefore provide new approaches to the treatment of a wide range of pathologies. Angiogenesis is a complex process in which the following events are believed to play a critical role:   

  • Proteolytic degradation of the extracellular matrix
  • Directed migration of endothelial cells
  • Proliferation of endothelial cells  
  • Deposition of new extracellular matrix
  • Formation of tubules and anastomosis of the newly formed vessels

Our 3D Human HUVECs Angiogenesis model is constructed using GFP-Tagged human umbilical vein endothelial cells (HUVECs) are co-cultured with RFP-Tagged supporting cells. GFP positive capillary like tubule formation can be monitored in real time under fluorescence microscope throughout the whole process of the experiment.

The 3D model is designed that the testing materials, i.e. compounds, conditioned media, or tissue explants, can be added into the system at any time, ranging from the onset of vasculogenesis to advanced angiogenesis. The resulting effect on tubule formation (tubular length, number of branches, etc.) can be monitored throughout the whole process under inverted fluorescence microscope.

Images
Left: 4x overlay image of HUVECs used in an endothelial  cell capillary tube formation assay. The cells were stained with calcein.Imaging was performed in the brightfield and GFP channels. Right: Co-culture system

Left: 4x overlay image of HUVECs used in an endothelial cell capillary tube formation assay. The cells were stained with calcein.Imaging was performed in the brightfield and GFP channels. Right: Co-culture system

Left: 4x overlay image of HUVECs used in an endothelial  cell capillary tube formation assay. The cells were stained with calcein.Imaging was performed in the brightfield and GFP channels. Right: Co-culture system

Left: 4x overlay image of HUVECs used in an endothelial cell capillary tube formation assay. The cells were stained with calcein.Imaging was performed in the brightfield and GFP channels. Right: Co-culture system

HUVECs growing in inserts

HUVECs growing in inserts

HUVECs growing in inserts

HUVECs growing in inserts