Product Details
Catalog Number: 5-4723-001
Applications: Gene Transfer
Type: Expression Vector
Format A: liquid
Downloads: Datasheet (pdf)
Downloads: MSDS (pdf)
Product Sizes
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5 µg$326.00Add to Cart

The pYSG-IBA123 vector allows the high-level expression of GST/Twin-Strep-tag®-fusion-proteins in yeast. The vector carries the copper inducible promoter (CUP1) for controlled high-level expression, the GST-tag for N-terminal and the Twin-Strep-tag® for C-terminal fusion of the recombinant protein as well as the URA3 auxotrophy marker for selection after transformation (do not use URA3 for selection during expression), the LEU2d auxotrophy marker for selection to increase plasmid copy number for expression (do not use LEU2d for selection after transformation). The expressed recombinant protein will be localized in the cytoplasm.