Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD3 Fab-TACS® Agarose Column Starter Kit allows label-free isolation of human CD3+ T cells from whole blood, buffy coat, PBMCs or other cell suspensions. CD3 is a co-receptor that associates with the T cell receptor and plays a role in T cell activation.
Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.
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- Philipp Boosz, et al. (2021). Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles Enable a Stable Non-Spilling Loading of T Cells and Their Magnetic Accumulation. Cancers, 13(16), 4143. doi: 10.3390/cancers13164143
- M Mühlberger, et al. (2019). Functionalization of T Lymphocytes With Citrate-Coated Superparamagnetic Iron Oxide Nanoparticles For Magnetically Controlled Immune Therapy. International Journal of Nanomedicine, 14:8421-8432. doi: 10.2147/IJN.S218488
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