CD45RA Fab-TACS® Agarose Column Starter Kit, human

Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD45RA Fab-TACS® Agarose Column Starter Kit allows label-free isolation of human CD45RA+ T cells from whole blood, buffy coat, PBMCs or other cell suspensions. CD45RA is an isoform of tyrosine phosphatase CD45 (leukocyte common antigen) that is expressed on different leukocytes including naïve and resting T cells, B cells as well as monocytes.

Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.

Advantages:

• Highly pure cells
• Reversible binding to the target
• Highly viable, non-activated cells
• Label-free cells due to low affinity Fab fragments

The kit contains:

• Strep-Tactin® TACS Agarose columns , 1 ml (2x)
• CD45RA Fab-Strep, human
• 10x Buffer CI
• Biotin stock solution
• TACS Column Adapter

For total cell numbers below 1 x 108, we recommend our Strep-Tactin® Magnetic Microbeads for free scalability.