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For fluorescent staining/FACS as well as for magnetic isolation of antigen-specific (CD8+) T cells.
Cell staining/FACS with fluorescent MHC I Streptamers: Combine MHC I-Streps with Strep-Tactin® PE or Strep-Tactin® APC
Cell isolation with magnetic MHC I Streptamers: Combine MHC I-Streps with Strep-Tactin® magnetic microbeads
Removal of Streptamers: Use Biotin to completely remove all labeling reagents from the stained/isolated cells.