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MHC I-Strep HLA-A*0201; WT-1(37-45) (VLDFAPPGA) is a recombinantly expressed MHC class I molecule that presents a peptide from Wilms tumor antigen 1 (WT1). Our MHC I Streptamer® approach is based on our Strep-tag® technology and facilitates reversible antigen-specific T cell labeling. A Twin-Strep-tag® fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin® backbone, resulting in the formation of MHC I Streptamers.
For multimerizing MHC I-Streps, Strep-Tactin® TACS Agarose columns, Strep-Tactin® Magnetic Microbeads or fluorophore-conjugated Strep-Tactin® can be used. This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements. The addition of biotin reverses the multimerization of MHC class I molecules, leading to their dissociation from the target cells. The resulting label-free cells are highly viable and fully functional.
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- Xinchao Wang, et al. (2010). Streptamer-Based Selection of WT1-Specific CD8+ T Cells for Specific Donor Lymphocyte Infusions. Immunotherapy, 38(11), 1066-1073. doi: 10.1016/j.exphem.2010.07.002
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