Product Details
Catalog Number: 6-8507-150
Applications: Cell Assays
Type: Cell Isolation-Separation
Storage: Lyophilized: 2-8°C; reconstituted: -80°C. Avoid multiple freeze-thaw cycles.
Shipping: Ambient
Format B: lyophilized
Species Reactivity: Mouse
Downloads: Datasheet (pdf)
Downloads: MSDS (pdf)
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CD19 Nano-Strep is a recombinantly expressed low affinity nanobody fused to a Twin-Strep-tag®. Due to its low affinity for the target, an increase in avidity is required to ensure sufficient binding to the cell surface. Combining Nano-Streps with a Strep-Tactin® backbone leads to the formation of complexes that stably bind to cells.

Nano-Streps were developed for Traceless Affinity Cell Selection (Nano-TACS®), which is based on our Strep-tag® technology.

Depending on the Strep-Tactin® backbone, the following applications are possible:

Fluorescent cell staining and isolation: Nano-Streps are combined with fluorophore-conjugated Strep-Tactin®. The resulting complex is suitable for fluorescent cell staining and sorting.

Affinity chromatographic cell isolation: Nano-Streps can be loaded in Strep-Tactin® TACS Agarose Columns that are suitable for affinity chromatographic cell isolation.

By addition of biotin, the labeling reagents dissociate mildly and rapidly from the selected target cells in all applications, yielding minimally manipulated cells for unbiased further use.